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The Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase From Human Fibriod Tissue
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The Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase from Human Fibriod Tissue

The research is aimed at investigating the effect of sodium citrate and reduced glutathione (GSH) on arginase from human fibriod tissue.

The research shows that arginase enzyme which was partially purified was found to have a specific interaction with its substrate.

The study shows that varying the substrate concentration at constant volume of Sodium citrate and Reduced glutathione (GSH) resulted into an Un-competitive inhibition and Competitive inhibition of enzymes respectively.

The results obtained from this study shows that the inhibition nature of arginase by different inhibitors of enzymes gives a promising approach to abnormal arginase activities in cell proliferation of uterine muscle which leads to fibroid.

Material Excerpt on the Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase from Human Fibriod Tissue

TABLE OF CONTENTS

PRELIMINARY PAGES

  • Title page
  • Approval page
  • Dedication
  • Acknowledgement
  • Table of Contents
  • Abstract

CHAPTER ONE

  • 1.0 Introduction and Literature Review
  • 1.1 Fibroid
  • 1.1.1 Location and classification of fibroid
  • 1.1.2 Pathogenesis and Pathophysiology
  • 1.1.2.1 Pathogenesis
  • 1.1.2.2 Pathophysiology
  • 1.1.3 Signs, Symptoms and Causes of fibroid
  • 1.2 Arginase
  • 1.2.1 Role of arginase in human body
  • 1.2.2 Aginase activities in Benign tumour
  • 1.2.3 Arginase II expression in benign and malignant prostate tissue
  • 1.3 Glutathione (GSH)
  • 1.3.1 Role of glutathione as an antioxidant
  • 1.3.2 Glutathione response to exercise
  • 1.4 Sodium citrate
  • 1.5 Aims and objectives

CHAPTER TWO

  • 2.0 Materials and Methods
  • 2.1 Materials
  • 2.1.1 Apparatus
  • 2.2 Methods
  • 2.2.1 Preparation of buffers and reagents
  • 2.3 Biochemical test
  • 2.3.1 Arginase assay
  • 2.3.2 Protein standard curve
  • 2.3.3 Effect of Glutathione (GSH)

CHAPTER THREE

  • 3.0 Results

CHAPTER FOUR

  • 4.0 Discussion, Conclusion and Recommendation
  • 4.1 Discussion
  • 4.2 Conclusion
  • 4.3 Recommendations

REFERENCES

CHAPTER ONE

1.1 Introduction

In this section, the Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase from Human Fibriod Tissue is discussed, with relevant and recent citations. As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the aim and objectives of the study. Others are significance of the study, scope of work, research hypothesis and questions, limitation of the study and definition of terms.

CHAPTER TWO

LITERATURE REVIEW

2.1 Introduction

This chapter focuses on the review of related literature. A literature review presents current knowledge, as well as theoretical and methodological contributions, related to the Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase from Human Fibriod Tissue. It documents the state of the art on the subject under study and provides a comprehensive survey of existing literature. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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