| Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat Project / Seminar Material Reference ID: PS-3024-TM |
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PRELIMINARY PAGES
CHAPTER ONE
- 1.0 Introduction
- 1.1 Aim of research
CHAPTER TWO
- 2.0 Literature Review
- 2.1 Physiochemical features of rhodanese
- 2.1.1 Mechanism of action
- 2.1.2 Assay methods for rhodanese
- 2.1.3 Mechanism of rhodanese catalysis
- 2.1.4 Structure
- 2.1.5 Amino acid composition
- 2.1.6 Active sites
- 2.2 Goat
- 2.3 Liver
- 2.3.1 Synthesis
- 2.3.2 Liver disease
- 2.3.3 Fetal blood supply
CHAPTER THREE
- 3.0 Materials and Methods
- 3.1 Materials used
- 3.2 Reagents used
- 3.3 Preparation of buffer and reagents used
- 3.4 Collection of sample
- 3.5 Preparation of tissue extract
- 3.6 Enzyme assay
- 3.7 Protein determination
- 3.8 Effect of temperature on rhodanese
- 3.9 Purification of rhodanese from crude extract
- 3.10 Determination of protein concentration and enzyme activities
CHAPTER FOUR
CHAPTER FIVE
- 5.0 Discussion, Conclusion and Recommendation
- 5.1 Discussion
- 5.2 Conclusion
- 5.3 Recommendations
REFERENCES
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The research is aimed at investigating the effect of temperature and heat stability of Rhodanese from the Liver of a local goat. This research shows the existence of rhodanese from a local goat liver homogenate. It was observed that the enzyme was partially purified by ammonium sulphate precipitation and dialysis. The specific activity of enzyme after partial purification using ammonium sulphate precipitation and dialysis was 4.39 U/mg. The effect of temperatures between 30 and 80?C on the rhodanese activity showed that optimum temperature for the enzyme was at 60?C. It was found that the enzyme (rhodanese) from a local goat liver was stable at 60?C, but was unstable at temperatures = 70oC.
Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat
1.1 Introduction
… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.
This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …
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