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Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat
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Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat


This page presents an excerpt of the available research material, including the Preliminary Pages, Table of Contents, Abstract, Chapters One to Five, and References. It provides a comprehensive overview of the study, enhancing readability and accessibility for students, and researchers seeking complete material on the topic stated above.


ACKNOWLEDGEMENT


I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




PRELIMINARY PAGES


CHAPTER ONE

  • 1.0 Introduction
  • 1.1 Aim of research

CHAPTER TWO

  • 2.0 Literature Review
  • 2.1 Physiochemical features of rhodanese
  • 2.1.1 Mechanism of action
  • 2.1.2 Assay methods for rhodanese
  • 2.1.3 Mechanism of rhodanese catalysis
  • 2.1.4 Structure
  • 2.1.5 Amino acid composition
  • 2.1.6 Active sites
  • 2.2 Goat
  • 2.3 Liver
  • 2.3.1 Synthesis
  • 2.3.2 Liver disease
  • 2.3.3 Fetal blood supply

CHAPTER THREE

  • 3.0 Materials and Methods
  • 3.1 Materials used
  • 3.2 Reagents used
  • 3.3 Preparation of buffer and reagents used
  • 3.4 Collection of sample
  • 3.5 Preparation of tissue extract
  • 3.6 Enzyme assay
  • 3.7 Protein determination
  • 3.8 Effect of temperature on rhodanese
  • 3.9 Purification of rhodanese from crude extract
  • 3.10 Determination of protein concentration and enzyme activities

CHAPTER FOUR

  • 4.0 Results

CHAPTER FIVE

  • 5.0 Discussion, Conclusion and Recommendation
  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendations

REFERENCES

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ABSTRACT


The research is aimed at investigating the effect of temperature and heat stability of Rhodanese from the Liver of a local goat. This research shows the existence of rhodanese from a local goat liver homogenate. It was observed that the enzyme was partially purified by ammonium sulphate precipitation and dialysis. The specific activity of enzyme after partial purification using ammonium sulphate precipitation and dialysis was 4.39 U/mg. The effect of temperatures between 30 and 80?C on the rhodanese activity showed that optimum temperature for the enzyme was at 60?C. It was found that the enzyme (rhodanese) from a local goat liver was stable at 60?C, but was unstable at temperatures = 70oC.




1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the aim and objectives of the study. Others are significance of the study, scope of work, Research hypothesis and questions, limitation of the study and definition of terms.…


CHAPTER TWO

LITERATURE REVIEW


2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …


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