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Phytochemical Sreening AntiInflamatory and Analgesic Analysis of Upaca Staudtil Leaves

Phytochemical Sreening, Anti-Inflamatory and Analgesic Analysis of Upaca Staudtil Leaves

Project / Seminar Material
Reference ID: PS-1245-TM

DEDICATION

This research material titled “Phytochemical Sreening, Anti-Inflamatory and Analgesic Analysis of Upaca Staudtil Leaves” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Industrial Chemistry, Book Authors and Profound Scholars of existing or related project material on “Phytochemical Sreening, Anti-Inflamatory and Analgesic Analysis of Upaca Staudtil Leaves” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.


Phytochemical Sreening, Anti-Inflamatory and Analgesic Analysis of Upaca Staudtil Leaves

CHAPTER ONE

1.1 Introduction

Uapaca staudtii is employed traditionally in the treatment of disease conditions such as wound, inflammation and diarrhea. Furthermore, there is no literature report on anti-inflammatory, analgesic as well as chemistry of the plant. Hence it is necessary to carry out research study to ascertain the potency and safety of this medicinal plant in the management of pains and inflammation associated with wound. Chemical constituents responsible for the activities may lead to potent or even novel Compounds.

As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Limitation of the study and Definition of technical terms.


1.2 Aim and Objectives of the Study

The aim of the study is to carry out a Phytochemical Sreening, Anti-Inflamatory and Analgesic Analysis of Upaca Staudtil Leaves. In achieving this aim, the following specific objectives were laid out as follows:

  1. To carry out chemical and anti-inflammatory studies of U. staudtii leaves in rodents.
  2. To carry out the phytochemical analysis of the ethanolic extract of U. staudtii leaves.
  3. To determine the median lethal dose (LD50) of the ethanolic extract in order to predict the dose and safety of the extract.
  4. To study the dose-response anti-inflammatory and analgesic effect of the extract and fraction of U. staudtii leaves.
  5. To investigate the possible mechanism of anti-inflammatory and analgesic effect.
  6. Purification and isolation of bioactive compounds responsible for these pharmacologic actions.
  7. Characterization of active constituents isolated using various spectroscopic techniques such as Ultraviolet (UV), Infrared (IR), Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS).

Preparation and Extraction of Plant Material

The leaves were destalked, washed, air dried and pulverized. The powdered plant material was weighed and about 2.8 kg was extracted cold with 22 L of 50% ethanol and shaken intermittently for 72 hours at room temperature. After 72 hours, the liquid extract concentrated in vacuo using rotary evaporator at 45oC. The extract was further concentrated to dryness in a water bath at 50oC and weighed. The dried extract (450 g) was stored in a refrigerator until use.

Phytochemical Screening

The phytochemical screening was carried out on the ethanol extract of U. staudtii leaves according to standard methods to preliminary identify the classes of bioactive compounds in the plant extract.

Test for Alkaloids

About 0.5 g of the extract was stirred with 5ml of 5% hydrochloric acid in a test tube placed on a steam bath at 100oC. The mixture was filtered and divided into three portions of 1ml each. The first portion of the filtrate was treated with few drops of Meyer’s reagent while the second portion was treated with Dragendorff’s reagent. Precipitation or turbidity with these reagents was taken as the preliminary evidence for the presence of alkaloids in the extract. The third portion of the filtrate was treated with few drops of picric acid; a yellow precipitation was taken as preliminary evidence for the presence of alkaloids (Harborne, 1998; Sofowora, 1993).

Test for Anthraquinones

  1. Free Anthraquinone:

    About 0.5 g of plant extract was treated with 2ml of benzene, filtered and 0.5 ml of 10% ammonia solution added and shaken. The presence of pink, red or violet colour in the ammonical layer indicated the presence of free hydroxy anthraquinones (Sofowora, 1993).

  2. Combined Anthraquinone:

    About 0.5 g of the extract was boiled with 2 ml of aqueous tetraoxosulphate (VI) acid and filtered while hot. The filtrate was shaken with 1ml of benzene.

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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