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Studies on the Leaf Spot Disease of Eggplant

Studies on the Leaf Spot Disease of Eggplant

Project / Seminar Material
Reference ID: PS-6235-TM

DEDICATION

This research material titled “Studies on the Leaf Spot Disease of Eggplant” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Agricultural Engineering (AE), Book Authors and Profound Scholars of existing or related project material on “Studies on the Leaf Spot Disease of Eggplant” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

ABSTRACT

Field, greenhouse and laboratory studies were carried out at the Department of Crop Science,University of Nigeria, Nsukka in order to evaluate the leaf spot disease of eggplant and its management with some botanicals. Field survey of diseased plants was conducted on eggplant farm. Solanum aethiopicum L. plants were sampled on every 1 m distance along the diagonal transects for disease incidence and severity. Pathogen isolation from severely infected leaves was carried out in the laboratory where the diseased leaves were plated on fresh Potato Dextrose Agar. Identification of the isolated fungi was carried out with the aid of identification scheme based on their cultural characteristics. Nursery preparation for raising eggplants used in the green house was also carried out on a sterilized soil. The five most abundant pathogens isolated were inoculated separately on the seedlings of eggplant in five replicates.

The experiment was arranged in a completely randomized design. Phytochemical contents of the four plant extracts were determined in the laboratory. An in vitro control of the organism responsible for the leaf spots was carried out using plant extracts at 0.030 g/ml, 0.060 g/ml, and 0.120 g/ml and 0.250 g/ml concentrations. The experimental design was a 9×4 factorial in a completely randomized design (CRD). The data were analyzed using analysis of variance (ANOVA). Means were later separated using Fisher’s least significant difference (F-LSD).

Helminthosporium infestans Dur. & Mont, Cladophialophora carrionii Trejos, Aspergillus niger van Tieghem, Rhizopus nigricans Ehrenb and Neurospora africana Huang & Backus were isolated from the diseased eggplant leaves. H. infestans recorded the highest percentage frequency (61.11 %) while A. niger had the lowest percentage frequency (5.56 %). H. infestans was pathogenic to eggplant seedlings. The Koch postulate test confirmed H. infestans as the causal organism of the spots symptoms. The qualitative phytochemical analysis on the test plants revealed the presence of tannin, soluble carbohydrate, hydrogen cyanide, steroids, flavonoids, alkaloids as well as glucosides in all the extracts analyzed. The effect of ethanolic extracts of the test plants significantly (Preduced the incidence and severity of the pathogen. Anti-fungal activity of seed extracts of G. kola on H. infestans was highest at 0.0120 g/ml concentrations. G. kola could be used as fungicide to manage leaf spot in eggplant because of its availability and eco-friendliness.


Studies on the Leaf Spot Disease of Eggplant

CHAPTER ONE

1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

Summary Headlines for Studies on the Leaf Spot Disease of Eggplant



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