Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria

Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria

Project / Seminar Material
Reference ID: PS-24231-TM

DEDICATION

This research material titled “Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Microbiology, Book Authors and Profound Scholars of existing or related project material on “Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

TABLE OF CONTENTS

PRELIMINARY PAGES


CHAPTER ONE

INTRODUCTION


    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES

    ABSTRACT

    Garlic (Allium sativum) is a medicinal plant mainly used as condiments and for stopping in different cooking. The use of higher plants and their extracts to treat infections is an ancient practice in traditional medicine. The study evaluates the Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria. In achieving this aim, the following specific objectives were laid out as follows to: identify the activity of garlic extracts on both Gram negative and Gram positive standard bacterial strains and compares their effect with the effect of some antibiotics and assess the anti-bacterial effect of garlic against standard isolates of S. aureus and E. coli. Four different solvents having different polarity were used to extract the bioactive compound from garlic. The Antibacterial activity of the crude extracts of garlic was evaluated against Standard isolates of S. aureus and E. coli by an agar diffusion method. The trial was done in triplicates. A Factorial Design with three factors was used. The treatment means were compared by a Student’s t- test with least significant difference (LSD) at 5% (P=0.05) and the data analysis was performed using mini tab statistical software package. In this experiment the non-polar chloroform had higher inhibition zone. The highest yield potential was obtained from water followed by ethanol, chloroform and petroleum ether respectively. E. coli were so susceptible than S. aureus to the extracts. Garlic could be used as effective antibacterial agent for human pathogenic bacteria. This study will be of immense benefit to Microbiologist, Biochemist and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work.


    Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria

    CHAPTER ONE

    1.1 Introduction

    Garlic (Allium sativum) is a medicinal plant mainly used as condiments and for stopping in different cooking (Nabeel, 2014). The use of higher plants and their extracts to treat infections is an ancient practice in traditional medicine. Many plants have been used because of their antimicrobial treats, which are chiefly synthesis during secondary metabolism of the plants. The herbal medicine may be in the form of powders, liquid or mixtures which may be row or boiled, ointments linings and incision (Jehan et al., 2011). Traditional medicine is the sum total of knowledge skills and practices based on theories, beliefs and experiences indigenous to different cultural that are used to maintain health, as well as to prevent, diagnose, improve or matters. This plant is multiplied by vegetative reproduction rather than by sexual reproduction (seed). Individual garlic cloves are planted and they each produce a bulb in which the cloves all have the same genetic makeup as the original clove (Paul, 2010).

    As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Limitations of the Study and Definition of technical terms.

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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