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Comparative Study of Disinfectant Efficiency of Ethanol Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus

Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus

Project / Seminar Material
Reference ID: PS-6761-TM

DEDICATION

This research material titled “Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Microbiology, Book Authors and Profound Scholars of existing or related project material on “Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

TABLE OF CONTENTS

PRELIMINARY PAGES


CHAPTER ONE

INTRODUCTION


    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES

    ABSTRACT

    Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms. Also (50%, 60%, 70%, 85% and 95%) of ethanol as well as (5%, 10%, 20%, 25%, and 30%) Phenolics were used.

    Differences in concentrations tested was because, the original concentrations of the disinfectants differs. After 24 hours of incubation at 370C, the results showed that all the disinfectants inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured around each well by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 30% Phenolics had the best efficiency against both test organisms and 5% bleach had a better effect on Staphylococcus aureus than Pseudomonas aeruginosa, while ethanol showed least sensitivity. 70% concentration gave the highest effect on Staphylococcus aureus as compared with Pseudomonas aeruginosa.


    Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus

    CHAPTER ONE

    1.0 Introduction

    Microorganisms are minute living things that individually are too small to be seen with the unaided eyes (Tortora et al, 2007). Though only a minority of microorganisms are pathogenic (disease producing), practical knowledge of microbes is necessary for medicine and related health sciences. For example hospital workers must be able to protect patients from common microbes that are normally harmless but pose a threat to the sick and injured.

    Thousands of people died in devastating epidemics; the cause of which was not understood. Entire families died because vaccination and antibiotics were not available to fight infection (Johnson and Case, 1995). This leads to scientific control of microbial growth. This began only about 100 years ago. It was Pasteur’s work on microorganism that led scientists to believe that microbes were a possible cause of diseases and need to be eliminated or destroyed. Some examples off these microbes are; Bacteria, fungi, viruses and protozoa etc (Tortora et al, 2007).

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

    Summary Headlines for Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus



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