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Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus

Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus

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DEDICATION

This research material, titled “Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Microbiology for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

INTRODUCTION

    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction
    • ⋮

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction
    • ⋮

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • ⋮
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES


    ABSTRACT

    Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms. Also (50%, 60%, 70%, 85% and 95%) of ethanol as well as (5%, 10%, 20%, 25%, and 30%) Phenolics were used.

    Differences in concentrations tested was because, the original concentrations of the disinfectants differs. After 24 hours of incubation at 370C, the results showed that all the disinfectants inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured around each well by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 30% Phenolics had the best efficiency against both test organisms and 5% bleach had a better effect on Staphylococcus aureus than Pseudomonas aeruginosa, while ethanol showed least sensitivity. 70% concentration gave the highest effect on Staphylococcus aureus as compared with Pseudomonas aeruginosa.


    Comparative Study of Disinfectant Efficiency of Ethanol, Bleach and Phenolics Against Pseudomonas Aeruginosa and Staphylococcus Aureus

    CHAPTER ONE

    1.0 Introduction

    Microorganisms are minute living things that individually are too small to be seen with the unaided eyes (Tortora et al, 2007). Though only a minority of microorganisms are pathogenic (disease producing), practical knowledge of microbes is necessary for medicine and related health sciences. For example hospital workers must be able to protect patients from common microbes that are normally harmless but pose a threat to the sick and injured.

    Thousands of people died in devastating epidemics; the cause of which was not understood. Entire families died because vaccination and antibiotics were not available to fight infection (Johnson and Case, 1995). This leads to scientific control of microbial growth. This began only about 100 years ago. It was Pasteur’s work on microorganism that led scientists to believe that microbes were a possible cause of diseases and need to be eliminated or destroyed. Some examples off these microbes are; Bacteria, fungi, viruses and protozoa etc (Tortora et al, 2007).

    …

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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