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Evaluation of Selected Agroallied Wastes as Carrier for Trichoderma Harzianum

Evaluation of Selected Agroallied Wastes as Carrier for Trichoderma Harzianum

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DEDICATION

This research material, titled “Evaluation of Selected Agroallied Wastes as Carrier for Trichoderma Harzianum” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Agricultural Engineering (AE) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Evaluation of Selected Agroallied Wastes as Carrier for Trichoderma Harzianum provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




ABSTRACT

This study was carried out to estimate the effectiveness of agro-allied wastes as carriers of the biological control organism, T. harzianum, by evaluating colonization and sporulation rate of each. The selected substrates were corn husk, Alstonia and Iron wood. The substrates were supplemented with 5, 10, 15, and 20% of rice bran, and control treatment to show the improved rate of colonization. All substrate used proved to be effective. The slowest mycelia colonization rate was from the Iron wood with 50% at 10% rice supplement by day-5. Alstonia and cornhusk substrates at 10% proved to be the best with a fast percentage colonization of 77.5 and 85.5% by day-5. This result were analysed using one way ANOVA at 5% probability level using Student-Newman-Keul’s (SNK).



Evaluation of Selected Agroallied Wastes as Carrier for Trichoderma Harzianum


1.0 Introduction

Trichoderma a fungus belonging to the division Ascomycota (Imperfecti fungi) and to the family Hypocreales. They occur worldwide and are commonly associated with roots, soil and plant debris (Howell et al., 2003). They could survive on other host such as rotten tomatoes and cultures of sawdust when the environment is favourable (Apabalza et al., 2000).

They live in symbiotic relationship such as competitions, antibiosis and mycoparatism. Trichoderma species have a complex mycoparatism process where they secrete cell-wall degrading enzymes (Whipps, 2011). In the cell wall chitin which a polymer include endochitin, chitinolytic which degrade the cell wall and metabolism of organism (Goku et al., 2000).

Trichoderma is an important phytopathogen antagonist against some soil fungi and plant parasitic organism (nematodes). Different strains have being developed such as TH 36, TH16 and has been proved to be effective antagonist of conidia of Colletotrichum musae (banana rot) and Rhizoctonia solani causal organism of seedling wilt in many plant (Apabalza et al., 2000; Kerry, 2000 and Myer, 2001).

The use of bactericide and fungicides (e.g. Aldrex T) and use of chemical fertilizer in crop protection have being in use over the years; recently this form of crop protection are being discouraged due to its implication on the environment (Chanidallan and Eliot, 2009).

The use of biological control has been on the rise and emphasis are being placed on it due to the fact that it is environmentally friendly (Whipps, 2001).

Trichoderma has been found to produce and provide better protection for crops such as cowpea by colonizing the plant root (Adekunle et al., 2004) and effective in suppression of pathogens by competing for space, nutrients and secretion of diffusible antibiotics (Howell, 2003).

Trichoderma has been used as fungicide against foliar disease of crops and its effectiveness against damping off. As biocontrol they act indirectly or directly through their mechanism help improve environmental conditions, plant growth and induce systemic resistant. (Benitez, 2004).

Test conducted using various species of Trichoderma has shown them to be effective in seed treatment; Trichoderma konigii, Trichoderma harziaum and Trichoderma virivde used to treat seedling against soil borne disease like Marcophonmia phaseolina (Adeknule et al., 2000). Good and improved the resistance of tomatoes to Meloidogyne incognita when cultured in 1.5% tomatoes extracts (Dabbat AA et al., 2006).

The production of Trichoderma for use in the field would require fast growth resulting in large biomass. Availability of cheap substrate and energy source is of importance.

The objective of this study is to determine:

  1. The suitability of selected agro allied waste as carrier for Trichoderma harzianum
  2. The rate of colonization and sporulation in selected agro allied waste.
  3. The effect of soil amendment on growth and yield of a selected vegetable − Celosia argentea.

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

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Defense Procedure for Agricultural Engineering Researchers


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Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


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During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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