Isolation and Characterization of Antibiotic Producing Actinomycetes in a Rhizosphere Environment

Isolation and Characterization of Antibiotic Producing Actinomycetes in a Rhizosphere Environment

Project / Seminar Material
Reference ID: PS-3038-TM

DEDICATION

This research material titled “Isolation and Characterization of Antibiotic Producing Actinomycetes in a Rhizosphere Environment” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Biochemistry, Book Authors and Profound Scholars of existing or related project material on “Isolation and Characterization of Antibiotic Producing Actinomycetes in a Rhizosphere Environment” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

INTRODUCTION

    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction
    • ⋮

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction
    • ⋮

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • ⋮
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES

    ABSTRACT

    This research work was carried out to isolation and characterization of antibiotic producing actinomycetes in rhizosphere environments using the standard microbiological method, crowded plate method, streak plate method and pour plate method. Starch casein agar and Nutrient agar were used for the characterization of the growth organisms and tests such as gram staining, starch hydrolysis, casein hydrolysis, lipid hydrolysis, citrate, methyl red, catalase and indole were carried out. After culturing only 5 out of the 13 isolates (from a total of 25 soil samples) showed visible growth and had antimicrobial activity on selected organisms(Stapylococus aureus, Bacillus subtilis and Escherichia coli), the total count for the colony forming units ranged from 3.9×106– 5.2×106.

    The five isolates gotten from this work had four of them from the genus Streptomycetes (denoted as B, F, H and M) and the other from the genus Nocardia (L). Isolate B was active against Escherichia coli with a zone of inhibition measuring 26mm, isolate F, H, L and M were active against Staphylococcus aureus with zones of inhibition measuring 8mm, 9mm16mm and 9mm respectively, while isolate B, F, H, L and M were active against Bacillus subtitis with zones of inhibition measuring 5mm, 11mm, 11mm, 19mm and 7mm respectively. This study shows that Streptomyces are the most prevalent antibiotic producing actinomycetes in the soil. Therefore antibiotics should be taken only when needed to avoid antibiotic resistance by certain organisms and stored at appropriate conditions (temperature and pressure) hence, further purification, elucidation, and characterization are recommended to know the quality and novelty and commercial values of antibiotics.

    Isolation and Characterization of Antibiotic Producing Actinomycetes in a Rhizosphere Environment

    CHAPTER ONE

    1.1 Introduction

    … As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.

    …

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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