Project Topics Seminar Topics Post UTME Nursing Exam Past Questions
Search Topic
PARKLYN
ERVICES
· RC: 2994849
Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn

Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn

@SparklynServices
WhatsApp Channel

DEDICATION

This research material, titled “Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Microbiology for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




PRELIMINARY PAGES


CHAPTER ONE

  • 1.0 Introduction
  • 1.1 Statement of the problem
  • 1.2 Aims/Objective of the Study
  • 1.3 Limitation of the Study
  • 1.4 Significance of the Study

CHAPTER TWO

  • 2.0 Literature Review
  • 2.1 Maize as Sweet Corn
  • 2.2 Types of Sugary Corn and Their Qualities
  • 2.2.1 White Corn
  • 2.2.2 Yellow Corn
  • 2.2.3 Bi-Coloured Corn
  • 2.2.4 Mlti-coloured corn
  • 2.2.5 Black Corn
  • 2.3 Sugery Extender and Super Sweet Type of Corn
  • 2.3.1 Yellow Corn
  • 2.3.2 White Corn
  • 2.3.3 Bio-Coloured Corn
  • 2.4 Uses of Maize
  • 2.4.1 Human Food
  • 2.4.2 Table 1: Nutritional Values of Maize
  • 2.4.3 Ornament
  • 2.4.4 Fodder
  • 2.4.5 Bio-Fuel
  • 2.5 Factors That can Enhance Corn Spoilage
  • 2.6 Some Diseases and Pests of Corn

CHAPTER THREE

  • 3.0 Materials and Methods
  • 3.1 Materials
  • 3.2 Methods
  • 3.2.1 Collection of Sample
  • 3.2.2 Sterilization of the Class Wears
  • 3.2.3 Preparation of Culture media
  • 3.2.4 Preparation of sample
  • 3.2.5 Plating Techniques
  • 3.3 Identification and Characterization of Isolate
  • 3.3.1 Culture Characteristics
  • 3.3.2 Colony Morphology
  • 3.3.3 Spore Staining
  • 3.3.4 Motility Test
  • 3.5 Biochemical Test
  • 3.5.1 Carbohydrate Assimilation Test
  • 3.5.2 Amino-Acid Assimilation Test
  • 3.5.4 Lipase Activity Test
  • 3.3.5 Fungi Identification

CHAPTER FOUR

  • 4.0 Results
  • 4.1 Identification
  • 4.2 Enumeration of Isolate
  • 4.2.1 Table IV: frequency of Visible Colonies
  • 4.2.2 Figure I: Histogram of Isolate Frequency
  • 4.2.3 Figure II: Colony Plates of Fungi Isolates

CHAPTER FIVE

  • 5.0 Discussion And Conclusion
  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendation
  • REFERENCES
  • APPENDIX



ABSTRACT

Fungi spoilage organisms are silently invading acidifying, fermenting, discoloring, and disintegrating microbes that render corn (zea mays) unpalatable and unsafe. Fungi spoilage is caused by two factors, (biotic) living which includes insects, birds, rodents and microorganisms and (non-biotic) non-living which includes temperature, humidity and time. Two samples of spoilt corn, red (treated) and white were taken from the store respectivly for investigation to ascertain the microorganism associated with the spoilage of the corn. Attention was focused on fungi. Different methods were used in the identification and five genera were isolated. Mucor spp occurred with a frequency of approximately 6%, Aspergillus spp 9%, Rhizopus spp 15%, Penicillium spp 33% and Fusarium spp. 36%. Fusarium spp was most frequent in occurrence in both samples.



Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn


1.0 Introduction

What food is more synonymous with summon than freshly picked corn on the cob? Corn grows in “ears, each of which is covered in rows of kernels that are then protected by the silk-like threads called “corn silk” and encased in a husk. Corn is known scientifically as Zea Mays. This moniker reflects its traditional name, Maize, by which it was known to the Native Americans as well as many other cultures throughout the world. Although we often associate corn with the colour yellow, it actually comes in host of different varieties featuring an array of different colors, including red, pink, black, purple and blue. Although corn is now available in markets years round, it is the locally grown varieties that you can purchase during the summer months that not only tastes the best but are usually the least expensive (Pitt and Hocking, et al., 2009).

From the time when primitive man began to cultivate crops and store food, spoilage fungi have demanded their tithe. Fuzzes, powders and slimes of white or black, green, orange, red and brown are signs of silently invading, acidifying, fermenting, discoloring and disintegrating microbes rending commodities unpalatable or unsafe (Pitt and Hocking 2002). Pitt and Hocking 2009, also stated that in a prophetic article warned of the danger from common spoilage fungi. Since 1960, a seemingly endless dream of pathogenic fungi discovered. On these ground alone, the statement “it is only a mould” is no longer acceptable to microbiologist, health inspectors or consumers. The demand for accurate identification and characterization of food spoilage fungi has become urgent.

This research work is focused on corn and its spoilage organisms. It set out to document current knowledge on the interaction of corn and fungi in the context of spoilage. Area covered are how the fungi is isolated using the suitable best media and identification of corn spoilage fungi. Mycologist develops preference and peculiarities of the type of fungi that are routinely grown. Media will affect colony morphology and color whether particular structures are formed or not, and may affect whether the fungus will be even in culture (Hunt et al, 2004).

1.1 Statement Of The Problem

Corn (Zea Mays) is a grain grown by farmers in various parts of the world especially in Africa. Its spoilage most times limits its availability to consumers and causes economic waste to farmers. Corn is mostly spoiled by fungi. The identification and characterization of these spoilage organisms will go a long way to profer solution on matter how little to this problem of corn spoilage.


1.2 Aims / Objective Of The Study

To microbiologically evaluate and identify fungi involved in corn spoilage using maize as case study.


1.3 Significance Of The Study

The study will help in the acquisition of the knowledge of fungi associated or involved in the spoilage of corn, factors that enhance spoilage of corn and the risk involved in the consumption of spoilt corn.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

Procedure for Accessing and Downloading the Complete Material in PDF or DOCX Format

Above is a preview excerpt of the full study on “Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn”. The complete material, including all five chapters, is available for download upon request.


To obtain the complete research material content, simply place an order by paying the specified project or seminar fee using the account details or electronic payment (E-payment) system provided below.


Seminar Material
₦3,000
Project Material
₦5,000

For Mobile Money (MoMo) and Researchers Outside Nigeria, Kindly Request Complete Material via WhatsApp.


Account Details - For USSD / POS Transfer

ACCT NAMESPARKLYN SERVICES
Zenith Bank PLC1222599051
MoniePoint (MFB)8030511988
Paycom (OPay)8030511988

–– or ––



After payment, send message containing your payment receipt to Sparklyn Services with the phone number displayed below.


Once payment is confirmed, the complete document will be delivered via WhatsApp or email in Microsoft Word (MS-Word) format.




You can get more research topics on Microbiology, if you did not see your preferred topic from the alternate list above.

Defense Procedure for Microbiology Researchers


In preparation for defending a project or seminar on Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


Page Content Headings - Isolation Identification and Characterization of Fungi Associated with the Spoilage of Corn

    Download Material (Docx)