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Isolation, Identification and Characterization of Microorganism Involved in the Contamination of Yoghurt

Isolation, Identification and Characterization of Microorganism Involved in the Contamination of Yoghurt

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DEDICATION

This research material, titled “Isolation, Identification and Characterization of Microorganism Involved in the Contamination of Yoghurt” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Microbiology for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Isolation, Identification and Characterization of Microorganism Involved in the Contamination of Yoghurt provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

INTRODUCTION

  • 1.1 Introduction
  • 1.2 Background of Study
  • 1.3 Statement of Problems
  • 1.4 Aim and Objectives of Study
  • 1.5 Significance of Study
  • 1.6 Scope of Study
  • 1.7 Limitations of the Study

CHAPTER TWO

LITERATURE REVIEW

  • 2.1 Introduction
  • 2.2 Theoretical Review of Yoghurt
  • 2.2.1 Starter Cultures in Yoghurt
  • 2.2.2 Composition of Yoghurt
  • 2.2.3 Types of Yoghurt
  • 2.2.3.1 Flavored Yoghurt
  • 2.2.3.2 Frozen Flavored Yoghurt
  • 2.2.3.3 Acidophilus Yoghurt
  • 2.2.3.4 Set Yoghurt
  • 2.2.3.5 Stirred Yoghurt
  • 2.3 Factors Affecting the Quality of Yoghurt
  • 2.3.1 Type of Milk
  • 2.3.2 Starter Cultures
  • 2.3.3 Heat Treatment
  • 2.3.4 Storage of Yoghurt
  • 2.4 Nutritional Value of Yoghurt
  • 2.5 Therapeutic effect of Yoghurt
  • 2.6 Stabilizers in Yoghurt
  • 2.7 Gum Arabic as Stabilizer on Yoghurt Quality

CHAPTER THREE

MATERIALS AND METHODS

  • 3.1 Introduction
  • 3.2 Study Area
  • 3.3 Utilized Materials and Equipments
  • 3.4 Preparation of Media
  • 3.5 Yoghurt Samples Collection
  • 3.6 Microbiological Analysis
  • 3.7 Identification of Fungal Isolates
  • 3.8 Purification of Isolates

CHAPTER FOUR

RESULTS AND DISCUSSION

  • 4.1 Introduction
  • 4.2 Results
  • 4.3 Discussion of Findings
  • 4.3.1 Determination of pH
  • 4.3.2 Enumeration of Bacterial, Fungal Isolates and the pH of the Yogurts
  • 4.3.3 Isolation and Identification of Organisms

CHAPTER FIVE

SUMMARY, CONCLUSION AND RECOMMENDATION

  • 5.1 Introduction
  • 5.2 Summary of Findings
  • 5.3 Conclusion
  • 5.4 Recommendation

REFERENCES


Isolation, Identification and Characterization of Microorganism Involved in the Contamination of Yoghurt

CHAPTER ONE

1.1 Introduction

Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized cooled to 43°c and are inoculated with known cultures of microorganisms referred to as starter cultures. The starter cultures may be a pure culture of a particular species of Lactobacillus or a mixed culture of Streptococcus thermophilus and Lactobacillus bulgaricus in a 1:1 ratio.

The coccus which is the Streptococcus thermophilus grows faster than the Rod which is the Lactobacillus bulgaricus and is primarily responsible for acid production while the rod adds flavor and aroma. The growth of these Microorganisms causes the transformation of milk’s sugar, lactose into lactic acid. This process gives yoghurt it’s texture. The associative growth of the two organisms results in acid production at a rate greater than that produced by them individually.

As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Limitation of the study and Definition of technical terms.

…

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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In preparation for defending a project or seminar on Isolation, Identification and Characterization of Microorganism Involved in the Contamination of Yoghurt, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.

Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.

Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.

During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.

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