Potential of Bacteria Isolated from Onion Root in Biogas Production

Potential of Bacteria Isolated from Onion Root in Biogas Production

Project / Seminar Material
Reference ID: PS-24312-TM

DEDICATION

This research material titled “Potential of Bacteria Isolated from Onion Root in Biogas Production” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Microbiology, Book Authors and Profound Scholars of existing or related project material on “Potential of Bacteria Isolated from Onion Root in Biogas Production” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

TABLE OF CONTENTS

PRELIMINARY PAGES


CHAPTER ONE

INTRODUCTION


    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES

    ABSTRACT

    Biogas is the mixture of gases produced by the breakdown of organic matter in the absence of oxygen, primarily consisting of methane and carbon dioxide. Biogas can be produced from raw materials such as agricultural waste, manure, municipal waste, plant material, sewage, green waste or food waste. Biogas is a renewable energy source. The study determines the Potential of Bacteria Isolated from Onion Root in Biogas Production Imo State Nigeria. In achieving this aim, the following specific objectives were laid out to identify the Isolated Bacteria from Onion Root during Biogas Production, isolate the Phosphate Solubilizing Bacteria (PSB) on Onion Root and carry out a Pathogenicity Test and Biochemical Features of PSB on Onion Samples. Onions are grown primarily for their use as food, adding flavors to food and taste. The crop is also used for processing such as pickling, freezing, dehydration and oil extraction. Young and healthy green onion leaves with their white bases are eaten raw in salads. The PSB effect on onion growth was investigated under greenhouse conditions. These Phosphate Solubilizing Bacteria (PSB) increased plant height (11.7 to 38.8%), the number of leaves (3 to 70%), the bulb diameter (6.4 to 64.5%), the average root length (2.6 to 44.7%), the fresh weight of the bulb (2.9 to 34.1%) and the dry weight of the bulb (1.5 to 40.6%) when compared to the negative control. This study will be of immense benefit to researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.


    Potential of Bacteria Isolated from Onion Root in Biogas Production

    CHAPTER ONE

    1.1 Introduction

    Biogas is the mixture of gases produced by the breakdown of organic matter in the absence of oxygen (anaerobically), primarily consisting of methane and carbon dioxide. Biogas can be produced from raw materials such as agricultural waste, manure, municipal waste, plant material, sewage, green waste or food waste. Biogas is a renewable energy source (Wikipedia, 2021). Onion (Allium cepa L.) is one of the important vegetable crops grown globally, which belongs to the family Liliaceae. It is subdivided into three groups: Allium cepa, Allium aggregation, Allium prolilum, which are all diploids (Boukary, 2012).

    As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitations of the Study and Definition of technical terms.


    1.2 Background of Study

    Onions are grown primarily for their use as food, adding flavors to food and taste (Megersa, 2017). The crop is also used for processing such as pickling, freezing, dehydration and oil extraction. Young and healthy green onion leaves with their white bases are eaten raw in salads. In addition to consumption, onions are produced for medicinal purposes such as prevention and treatment of blood and heart diseases (Cheema et al., 2003). The crop is also a rich source of Vitamin C and E (Elassi, 2007) and in Uganda; onions contribute to household income especially in highland areas where they are grown purposely for sale (Bua et al., 2017). In India, onion is being grown in an area of 0.83 million hectares with production of 13.57 million tonnes and the productivity is 16.30 ton ha-1 which is low. Maharashtra is the leading onion producing state followed by Karnataka, Gujarat etc (FAO et al., 2018).

    In peri-urban areas of central Uganda, onion is becoming a major spice and vegetable produced by farmers in wetland areas. Consequently, strong help for smallholder producers to achieve potential yields to boost their income and secure their livelihood by providing high yielding and adapted onion genotype is critical. However, the present challenge in most onion growing areas is lack of well-adapted onion genotypes. As a result, varietal information to improve onion yield in production areas is inadequate. Additionally, research has not established a breeding program to develop improved varieties or even evaluated existing onion genotypes imported from outside Uganda to guide growers in selecting best yielding onion varieties to use. Evaluation of onion genotypes for their yield performance is considered as one approach to use in obtaining high yielding and adapted onion genotype.

    Therefore, in Imo State Nigeria where the research was carried out, the activities that was conducted is to know the Potential of Bacteria Isolated from Onion Root in Biogas Production.


    1.3 Statement of Problems

    Investigation reveals that onion production remains as low input and output crop with a national average yield of 5 ton ha-1 (FAO, 2012). However, the yield of the crop is far below the world average (19.7 ton ha-1) despite its being year- round production (FAO, 2012). The low onion yield in Uganda is partly attributed to farmers' exchange of planting materials (bulbs), they rarely purchase improved and well-adapted genotypes.

    Furthermore, due to limited research on commercial production of onion, farmers rarely use improved production practices (Bewuketu et al., 2016). Common pests such as thrips decrease yield in the absence of timely pesticide application or resistant onion genotypes (Harsimran et al., 2015). Similarly, the ineffective post-harvest handling and storage practices used by farmers also contribute to about 25% losses (Harsimran et al., 2015). An ethnobotanical survey tells of the Fennoscandian multiplying onions as being a crop with reliable harvest, excellent storage ability, and good taste. Increased cultivation of this material on both household and commercial-scale should be possible (Leino et al., 2018).


    1.4 Aim and Objectives of Study

    The aim of the study is to determine the Potential of Bacteria Isolated from Onion Root in Biogas Production Imo State Nigeria. In achieving this aim, the following specific objectives were laid out as follows:

    1. To isolate the Phosphate Solubilizing Bacteria (PSB) on Onion Root
    2. To carry out a Pathogenicity Test and Biochemical Features of PSB on Onion Samples
    3. To identify the Isolated Bacteria from Onion Root during Biogas Production

    1.5 Significance of Study

    This study will be of immense benefit to researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.


    1.6 Scope of Study

    The study focuses on the Potential of Bacteria Isolated from Onion Root in Biogas Production Imo State Nigeria.


    1.7 Limitations of the Study

    During the course of this study, many things militated against its completion, some of which are:

    1. Time Constraint: The time frame given to accomplish this project was very short due to school academic calendar and it was carried out under pressure which made the researcher not to implement some necessary features.
    2. Research material: availability of research material is a major setback to the scope of the study.
    3. Frequent power failure: This made the researcher append more money on fuel to ensure sustainable power.
    4. Financial Constraint: Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet).

    1.8 Definition of Technical Terms

    Heavy: having great weight; also characterized by mass or weight.

    Metal: Metals are opaque, lustrous elements that are good conductors of heat and electricity. Most metals are malleable and ductile and are, in general, denser than the other elemental substances

    Adverse Effect: An adverse effect is an undesired harmful effect resulting from a medication or other intervention, such as surgery.

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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