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Prevalence of Multidrug Resistant Livestock Associated Staphylococcus Aureus Isolated From Nasal Passage of Healthy Cattle

Prevalence of Multidrug Resistant Livestock Associated Staphylococcus Aureus Isolated From Nasal Passage of Healthy Cattle

Project / Seminar Material
Reference ID: PS-649-TM

DEDICATION

This research material titled “Prevalence of Multidrug Resistant Livestock Associated Staphylococcus Aureus Isolated From Nasal Passage of Healthy Cattle” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Microbiology, Book Authors and Profound Scholars of existing or related project material on “Prevalence of Multidrug Resistant Livestock Associated Staphylococcus Aureus Isolated From Nasal Passage of Healthy Cattle” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

TABLE OF CONTENTS

PRELIMINARY PAGES


CHAPTER ONE

INTRODUCTION


    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES


    Prevalence of Multidrug Resistant Livestock Associated Staphylococcus Aureus Isolated From Nasal Passage of Healthy Cattle

    CHAPTER ONE

    1.0 Introduction

    1.1 Background to the Study

    Cattle are large bodied ruminants that feed on pastures and forages or fodder. In Nigeria, cattle are reared primarily for meat which is a veritable source of protein for humans, and for milk (Arowolo et al., 2013). Cattle rearing in Nigeria is an old occupation which is traditionally practiced by Fulanis and Shuwa Arabs in northern Nigeria with an extremely few local villages in the southern Nigeria (Erebor, 2003).

    In people, the diseases brought on by S. aureus can be partitioned into these three sorts in general; shallow sores, (for example, surgical site and wound contaminations), life and systemic undermining factors, (for example, osteomyelitis, endocarditis, pneumonia, mind abscesses/wounds, bacteraemia and meningitis), then toxinoses, (for example, poisonous stun disorder, sustenance harming and singed skin disorder (Alo et al., 2013; Aires de Sousa et al., 2004; Lowy, 2003). The sign of staphylococcal contamination are the boils that contain discharge which is made up of dead neutrophils, dead and living microbes, tissue (necrotic), the lysed host substance and bacterial cells.

    The immunocompetent hosts, as a rule, effectively clear the disease and deplete the ulcer, though for the immunocompromised and sporadically for a sound individual, the contamination might advance to a more profound tissues and turn into a conceivably lethal intrusive contamination (Norvick, 2006). S. aureus is likewise known to colonize and contaminate both pets and animals, including pooches, felines, rabbits, stallions, steers and pigs (Morgan, 2008). A noteworthy concern is the nearness of methicillin safe S. aureus (MRSA) in pets and domesticated animals, as these may fill in as repositories for human colonization, an illustration is MRSA ST398 from pigs (Weese, 2010).

    One purpose behind the proceeding with essential part of S. aureus in illness is its inclination and propensity to wind up distinctly impervious to antimicrobial (Waldvogel, 2000). S. aureus is presently the main general reason for nosocomial diseases and, as more patients are dealt with outside the healing center setting, is an expanding worry in the group (CDC NNIS System, 2001; Diekema, 2001).

    The time of medication development and its execution in human and creature wellbeing and horticulture was started by the revelation of anti-infection agents over 70 years prior. These disclosures were powerful against organisms consequently were viewed as effective against pathogenic microorganisms however this achievement was fleeting as they were tempered with in all cases by the rise of resistant microorganisms (D’Costa et al., 2011). A standout amongst the most relentless issues confronted by human services benefits far and wide is the expanding pervasiveness of antimicrobial resistance. This resistance is broadly perceived as a noteworthy general wellbeing danger and this issue is aggravated by a consistent reducing of the quantity of new specialists (antimicrobials) entering the clinical practice (D.H, 2000).

    There is an expanding worry that some less-alarming infections which were effortlessly treated are currently turning out to be progressively hard to treat and ailments created by microscopic organisms which are impervious to antimicrobial agents may set aside a more drawn out time of opportunity to treat successfully (Butler et al., 2006). In spite of the fact that the issue of multidrug resistance has pulled in the consideration of medicinal services administrations and the overall population, rates of antimicrobial resistance among healing center and group pathogens have expanded alarmingly amid the previous decade (NNIS, 2001).


    1.2 Statement of the Problem

    In Nigeria, cattle are reared primarily as a source of meat. According to Kuehnert et al. (2006), Lowy (1998, 2003), Onanuga & Temedie (2011), Vandenbergh & Verbrugh (1999), Williams (1963), the nares and the skin of humans and animals may be considered as ecological niche for S. aureus colonization but this colonization does not frequently result into infection thereby tagging the bacterium a normal flora of these body parts. S. aureus colonizes the nares and the skin but if there is an abrasion, lesion or wound in these parts, S. aureus may migrate into the body or blood and cause infections.

    These infections are called opportunistic (staphylococcal) infections. Compared to other pathogens, S. aureus has a high tendency and proneness to become resistant to antimicrobials (Weese, 2010). This fact, coupled with the constant abuse of drugs and lack of control in the sales of antibiotics contributes to the increasing problem in multidrug resistance of S. aureus including methicillin and vancomycin which is considered as the first line of treatment against methicillin resistant Staphylococcus aureus (MRSA). Cuny et al. (2015), Fluit (2012), Johnson (2011) and Morgan (2008) established that methicillin resistant S. aureus (a multidrug resistant organism) may not only be a nosocomial and community acquired infection but it could also be a zoonotic infection as it can be transmitted from animal to human.

    The presence of multidrug resistant S. aureus in the nares of cattle poses a threat to cattle herders, butchers, beef retailers/handlers and consumers as these cattle are frequently asymptomatic carriers hence are considered ‘healthy’. This study may evaluate the prevalence of multiple antibiotic resistant livestock associated S. aureus and suggest possible control to diseases caused by multiple antibiotic resistant livestock associated S. aureus.


    1.3 Aim and Objectives of the Study

    The main aim of the study is to evaluate the prevalence of multidrug resistant livestock associated S. aureus and suggest possible control to staphylococcal infections in humans caused by livestock associated S. aureus using the antibiogram of the isolates.

    The specific objectives are to:

    1. Isolate and identify S. aureus from nasal passage of healthy cattle by Gram staining and biochemical tests (catalase test, slide coagulase test and fermentation of mannitol);
    2. Determine the antibiogram of the isolates and calculate the Multiple Antibiotic Resistance Index (M.A.R.I) using the antibiogram of the isolates;
    3. Determine the minimum inhibitory concentration (µg/disc) of the S. aureus isolates to vancomycin;
    4. Determine the minimum inhibitory concentration (µg/ml) of the isolates to flucloxacillin as a test for methicillin resistant S. aureus (MRSA) and vancomycin
    5. Determine the synergistic, antagonistic or additive effect of two antibiotics to suggest possible control of staphylococcal infections in human caused by multidrug resistant livestock associated S. aureus.

    1.4 Research Questions

    The study came up with research questions so as to be able to ascertain the above stated objectives. The specific research questions for the study are stated below as follows:

    • Can S. aureus be isolated and identified from nasal passage of healthy cattle?
    • How is the antibibiogram of S. aureus isolates determined and how are the Multiple Antibiotic Resistance Index of the isolates calculated using the antibiogram of the isolates?
    • How is the minimum inhibitory concentration (µg/disc) of S. aureus determined?
    • How is the minimum inhibitory concentration (µg/ml) of S. aureus isolated from nasal passage of healthy cattle to flucloxacillin and vancomycin determined?
    • Are the effects of the combined antibiotics synergistic, additive or antagonistic?

    1.5 Significance of the Study

    The following are the significance of this study:

    1. This result may create more awareness on the danger of multidrug resistant S. aureus in ruminant flocks.
    2. This result may educate the general public on the effect of negligence of multidrug resistant S. aureus in cattle and its effect in the consumption of undercooked beef.
    3. The result may suggest possible control of staphylococcal infections in humans caused by livestock associated S. aureus.

    1.6 Justification for the Study

    The study may provide a more recent data on multiple antibiotic resistant S. aureus and suggest possible control of livestock associated staphylococcal infections in human.

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

    Summary Headlines for Prevalence of Multidrug Resistant Livestock Associated Staphylococcus Aureus Isolated From Nasal Passage of Healthy Cattle



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