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Diagnostic Tests and Epidemiological Probes for Prawn Viruses in Nigeria

Diagnostic Tests and Epidemiological Probes for Prawn Viruses in Nigeria

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Reference ID: PS-2013-TM

DEDICATION

This research work titled "Diagnostic Tests and Epidemiological Probes for Prawn Viruses in Nigeria" is dedicated to God for his enabling grace and to all computer enthusiasts who help to make life a pleasant experience.

ACKNOWLEDGEMENT

I owe my indebtedness to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Fishery and Aquaculture, Book Authors and Profound Scholars of existing/related research material for your moral support that facilitated the successful completion of my (Tertiary Institution level). I am grateful to God Almighty and my parent for their financial support in my career. I really appreciate you all for everything, Thank you very much.


Diagnostic Tests and Epidemiological Probes for Prawn Viruses in Nigeria

CHAPTER ONE

1.0 Introduction

1.1 Background To The Study

Viral diseases have been seriously impacting the sustainability and economic success of prawn aquaculture as they have been resulting in heavy mortalities (Carr et al, 1996). About 20 different prawn viruses have been reported, which have been implicated in mass mortalities in cultured prawn, such as White spot syndrome virus (WSSV), yellow head virus (YHV), infectious hypodermic and hematopoietic necrosis virus (IHHNV) and Taura syndrome virus (TSV). IHHNV, Penaeus monodon densovirus (PmoDNV), formerly referred to as hepatopancreatic parvovirus (HPV) and lymphoidal parvovirus (LPV) associated with parvoviral infections in prawns (Felix & Devarag, 1993). Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is one of the major viral pathogens of prawn worldwide, which has resulted in severe mortalities of up to 90 % in cultured prawn from Nigeria and hence designated Penaeus stylirostris densovirus (PstDNV).

Infectious hypodermal and hematopoietic necrosis virus is distributed in prawn culture facilities worldwide. It causes large economic loss to the prawn farming industry (Anon, 2009). Our knowledge about the natural reservoirs of Infectious hypodermal and hematopoietic necrosis virus is still scarce. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) of prawn, one of the major viral pathogens of prawns has recently been classified as Penaeus stylirostris densovirus (PstDNV) under the family Parvoviridae and is a DNA virus.

It has been reported to cause Runt deformity syndrome (RDS) in the two species of prawn namely P. (Litopenaeus) vannamei and P. monodon which is a chronic, non-lethal disease (Kalagayan, 1991). Like all other parvoviruses, Infectious hypodermal and hematopoietic necrosis virus do not encode a DNA polymerase and depend on host cells for DNA replication and multiplication (Bell et al, 1988). Therefore, they need rapidly proliferating cells of the host for their replication. The target organs for Infectious hypodermal and hematopoietic necrosis virus infection include tissues of ectodermal (cuticular epidermis, hypodermal epithelium of the fore and hind gut, nerve cord and nerve ganglia) and mesodermal (hematopoietic organs, antennal gland, tubule epithelium, gonads, lymphoid organ, connective tissue and striated muscles) origin. The virus does not infect organ systems of endodermal origin (i.e., hepatopancreas, mid-gut epithelium, anterior mid-gut caecum or posterior midgut caecum).

Infectious hypodermal and hematopoietic necrosis virus infection has been demonstrated in all life stages of prawn species including eggs, larvae, postlarvae (PL), juveniles and adults. PL and juvenile prawns are more susceptible to Infectious hypodermal and hematopoietic necrosis virus infection than adults due to the presence of actively dividing cells (Anon, 2009). Infectious hypodermal and hematopoietic necrosis virus-infected females fail to develop embryos and also fail to hatch the eggs (Anon, 2009). This virus is highly species-specific, particularly in terms of gross manifestations. Due to the importance of prawn culture, the availability of easy and rapid methods that allow early diagnosis is essential for routine monitoring of the animal health status and to restrain further disease outbreaks (Pantoja et al, 1999). Traditionally, the diagnosis of Infectious hypodermal and hematopoietic necrosis virus infection is by examining histological sections wherein prominent Cowdry type A, eosinophilic, intranuclear inclusion bodies surrounded by marginated chromatin in hypertrophied nuclei of cells is seen in tissues of ectodermal and mesodermal origin (Carr et al, 1996). These inclusion bodies of Infectious hypodermal and hematopoietic necrosis virus can be confused with the developing inclusion bodies.


1.2 Statement Of The Problem

Viral pathogen of prawns has resulted in mass mortalities in prawn culture worldwide. In addition, it also causes abnormal deformities of cuticle, abdominal segments, tail fan and rostrum, wrinkled antennal flagella, bubble-heads along with wide size variations and reduced growth that finally affects the quality of the commodity prawn. Several important issues related to the process of infection, propagation and interaction of prawn viruses with hosts at the cellular and molecular level, remain to be elucidated.

Studies are needed as well, to determine the true risks of the viral infections on wild populations of prawns. Since Infectious hypodermal and hematopoietic necrosis virus is widely distributed in prawn culture facilities throughout the world, this study will focus on its diagnosis and its epidemiological probes. Recent reports show that genetic diversity of Infectious hypodermal and hematopoietic necrosis virus is not uniform across geographic regions. For investigation on mutations within a geographic area or individual hosts, genome sequencing is the method of choice.


1.3 Aim and Objectives Of The Study

The aim of the study is to examine the Diagnostic Tests and Epidemiological Probes for Prawn Viruses in Nigeria. In achieving this aim, the following specific objectives were laid out as follows:

  1. To identify the diagnostic tests for Infectious hypodermal and hematopoietic necrosis virus of prawns.
  2. To examine the epidemiology of Infectious hypodermal and hematopoietic necrosis virus of prawns.
  3. To examine the mortality rates of prawns infected with Infectious hypodermal and hematopoietic necrosis virus.

1.4 Research Questions

The study came up with research questions so as to be able to ascertain the above stated objectives. The specific research questions for the study are stated below as follows:

  • What are the diagnostic tests for Infectious hypodermal and hematopoietic necrosis virus of prawns?
  • What is the epidemiology of Infectious hypodermal and hematopoietic necrosis virus of prawns?
  • What is the mortality rates of prawns infected with Infectious hypodermal and hematopoietic necrosis virus?

1.5 Significance Of The Study

The following are the significance of this study:

  1. The outcome of this study will reveal various approaches to the diagnosis of Infectious hypodermal and hematopoietic necrosis virus in infected prawn. It will also educate on the epidemiology and mortality rate occasioned by Infectious hypodermal and hematopoietic necrosis virus in prawns.
  2. This research will be a contribution to the body of literature in the area of the effect of personality trait on student’s academic performance, thereby constituting the empirical literature for future research in the subject area.

1.6 Scope Of The Study

This study will cover procedures and investigations into the diagnostic tests and epidemiological probes for viruses in prawn in Nigeria with specific emphasis on Infectious hypodermal and hematopoietic necrosis virus.


1.7 Limitation Of Study

During the course of this study, there were some problems encountered which stood as limitations to the research work. Some of the limitations include:

  1. Time Constraint:

    The time frame given to accomplish this project was very short due to school academic calendar and it was carried out under pressure which made the researcher not to implement some necessary features.

  2. Financial Constraint:

    Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet, questionnaire and interview).

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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