Isolation and Characterization of Bacteria for Bioenzymes Production using Potato Peels

Isolation and Characterization of Bacteria for Bioenzymes Production using Potato Peels

Project / Seminar Material
Reference ID: PS-24080-TM

DEDICATION

This research material titled “Isolation and Characterization of Bacteria for Bioenzymes Production using Potato Peels” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Microbiology, Book Authors and Profound Scholars of existing or related project material on “Isolation and Characterization of Bacteria for Bioenzymes Production using Potato Peels” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.

TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

INTRODUCTION

    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction
    • ⋮

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction
    • ⋮

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • ⋮
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES

    ABSTRACT

    The study examines the Isolation and characterization of bacteria process for bioenzymes production using potato peels. In achieving this aim, the following specific objectives were laid out to identify the isolation process of bacteria for potato peels bioenzymes production, examine the characterization of bacteria process for potato peels bioenzymes production, and evaluate the measure of the potato peels bioenzyme efficacy. Potato (Solanum tuberosum L.) is an important crop in Nigeria, it is a primary source of starch for vegetarian diet. The wastes coming out from potato plants in the form of liquid and solid arise from peeling, trimming, slicing, cleaning, and rinsing operations which creates pollution problem. Data used in this work is derived primary from secondary sources. The methodology therefore, is basically documentary in nature. This involves learning new facts and principles through the study of documents and records. The documents and records include journals, textbooks, official publications etc. By putting together logically evidence derived from documents and records. Conclusion which either established facts hitherto unknown or sound generalization can be obtained. This study will be of immense benefit to microbiologist and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work.

    Isolation and Characterization of Bacteria for Bioenzymes Production using Potato Peels

    CHAPTER ONE

    1.1 Introduction

    Potato (Solanum tuberosum L.) is an important crop in Nigeria. It is a primary source of starch for vegetarian diet. In Nigeria, it was cultivated in an area of about 1.83 million hectares with production of 34.39 Million Tonne in 2008-09, whereas in Gujarat in the same year it was cultivated in 57,000 hectares with production of 4.21 Million Tonne (www. agricoop.gov.in). There are about 35 potato processing units in Gujarat foods, Rajkot is one of the biggest units (www.potatopro.com). This unit was visited by the staff members of scheme, to get information about the utilization of potato peel a waste product of wafer making unit. The capacity of the plant is about 150 tonnes potato per day, and potato peels production is about 30 tonnes potato peel at 200g / kg potato. Presently the potato peels are mainly used as a composting material in farm as well as selling for the cattle feed (according to Balaji foods (P.) Ltd, Rajkot).

    Most Bacillus and some other species of bacteria have a wide range of hydrolytic enzyme systems and are often capable of utilizing the organic matter consisting of complex mixtures; moreover, with the exception of the certain group of bacteria, they are harmless microbes which are included in the groups of organisms generally recognized as saprophytes. These organisms are easy to grow and require no expensive growth factors. They are able to produce extra cellular enzymes in high concentrations which can isolate. The Bacillus will also be tried for the extraction of extra cellular enzymes from potato peels. The extra cellular enzymes are used for a variety of different purposes, including starch modification in the paper & pulp industries, brewing, pharmaceutical industries, leather production industries etc. The wastes coming out from potato plants in the form of liquid and solid arise from peeling, trimming, slicing, cleaning, and rinsing operations which creates pollution problem.

    As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitations of the Study and Definition of technical terms.

    1.2 Statement of Problems

    Investigation reveals the problem the Isolation and characterization of bacteria for bioenzymes production using potato peels which entails that cellular enzymes are used for a variety of different purposes, including starch modification in the paper & pulp industries, brewing, pharmaceutical industries, leather production industries etc. The wastes coming out from potato plants in the form of liquid and solid arise from peeling, trimming, slicing, cleaning, and rinsing operations which creates pollution problem.

    1.4 Aim and Objectives of Study

    The aim of the study is to examine the Isolation and characterization of bacteria process for bioenzymes production using potato peels. In achieving this aim, the following specific objectives were laid out as follows:

    1. To identify the isolation process of bacteria for potato peels bioenzymes production
    2. To examine the characterization of bacteria process for potato peels bioenzymes production
    3. To evaluate the measure of the potato peels bioenzyme efficacy

    1.5 Significance of Study

    This study will be of immense benefit to microbiologist and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.

    1.6 Scope of Study

    The study focuses on the Isolation and characterization of bacteria process for bioenzymes production using potato peels in Gada Local Goverment Area, Sokoto State.

    1.7 Limitations of the Study

    During the course of this study, many things militated against its completion, some of which are:

    1. Time Constraint: The time frame given to accomplish this project was very short due to school academic calendar and it was carried out under pressure which made the researcher not to implement some necessary features.
    2. Research material: availability of research material is a major setback to the scope of the study.
    3. Frequent power failure: This made the researcher append more money on fuel to ensure sustainable power.
    4. Financial Constraint: Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet).

    1.8 Definition of Terms

    Potato: Is a starchy tuber of the plant Solanum tuberosum

    Machine: It’s an apparatus using mechanical power and having several parts, each with a definite function and together performing a particular task

    …

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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