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The Physicochemical Properties of Honey

The Physicochemical Properties of Honey

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DEDICATION

This research material, titled “The Physicochemical Properties of Honey” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on The Physicochemical Properties of Honey provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




PRELIMINARY PAGES


CHAPTER ONE

INTRODUCTION


    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES



    ABSTRACT

    Honey is a nutritious product that is produced by honey bees. Its composition depends highly on the type of flowers utilized by the bee as well as the climatic conditions. The purpose of the research work was to determine the quality of Nigerian honey in terms of physicochemical properties, and verify their compliance with international standards. The physicochemical property of the honeys [moisture, electrical conductivity, pH, HMF, acidity (free, total and lactones), reducing sugars, apparent sucrose, insoluble material, ash, and diastase activity] was determined. The moisture shows values of 14.21±0.18 % and 16.59±0.12%. The water insoluble material content ranged between 0.01 ± 0.03 to 0.67 ± 0.07%. The reducing sugars were between 66.3 ± 0.37 and 88 ± 0.23 %, mean percentages of apparent sucrose were 6.54 ± 0.23%. Honey samples showed an appropriate diastase number with an average of 12.35 ± 0.24, and their HMF content ranging from 5.65 ±0.37 to 37.42 ± 0.55 mg/kg, Conductivity values ranged from 10 ± 0.54 to 606 ± 2.45 µS / cm. The ash content ranged from 0.019 ± 0.01% to 0.37 ± 0.05%. All honeys were acidic, having a pH in the range 3.7 ± 0.03– 4.78 ± 0.05. The average values for free acidity in samples were between 10 ± 0.22 and 25 ± 0.5 meq / kg. Lactonic acidity ranges were from 2 ± 0.15 to 5 ± 0.15 meq / kg. Total acidity ranged from 12 ± 0.37 to 30 ± 0.65 meq / kg. All the samples were found to meet all major national and international honey specifications. This study will be of immense benefit to Agricultural Engineers, Veterinary Doctors, and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.



    The Physicochemical Properties of Honey


    1.1 Introduction

    Honey is defined as the natural sweet substance produced by honey bees from nectar of blossoms or from secretions of living parts of plants or excretions of plant sucking insects on the living part of plants, which honey bees collect, transform and combine with specific substances of their own, store and leave in the honey comb to ripen and mature (Mendes et al., 1998). Honey is a very important energy food and is used as an ingredient in hundreds of manufactured foods, mainly in cereal based products, for its sweetness, color, flavor, caramelization and viscosity (Rodriguez et al., 2004).

    As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Limitations of the Study and Definition of technical terms.


    1.2 Background of Study

    Honey is as old as written history dating back to 2100 BC where it was mentioned Sumerian and Babylonian cuneiform writings, the Hittie code and then sacred writings of India and Egypt it is presumably even older than that. In ancient days, honey has been used not only in food and beverages, but also to make cement in furniture polishes and varnishes and for medicinal purposes. And of course, bees perform vital senlice of pollinating fruits, legumes and other types of food producing plants in the course of their business of honey production. Honey was pronounced in English (hnni) is a sweet food made by bees using nectar from flowers. The variety produced by honey bees i.e. (GenusApis) is the most commonly referred to and is the type of honey collected by bee keepers and consumed by human. Honey produced by other bees and insects has distinctly different properties. Honey bees transform nectar into honey by a process of regurgitation, and store it as a primary food source wax honey combs inside the beehive. Beekeeping practices encourage over production of honey so the excess can be taken from the colony. Honey acts its sweetness from the monosaccharides fructose and glucose and has approximately the same relative sweetness as that of granulated sugar. It has attractive chemical properties for baking and distinctive flavours that leads some people to prefer it over sugar and other sweetness. Most microorganisms do not grow in honey because of its low water activity (9w) of 0.6. However, honey sometimes contains dormant endospores of the bacterium clostridium botulinum which can be dangerous to infants, as the endospores can transform into toxin producing bact Honey has a long history of human consumption, and is used in various foods and beverages as a sweetener and flavouring. It also has a role in religion and symbolism, flavours of honey varies based on the available. It is also used in various medicinal traditions to treat ailments. The study of pollens and spores in raw honey (mellissopalynology) can determine the floral source of honey. Because bees carry an Electrostatic charge and can attract other particles, the same techniques of melissopalynology can be used in area environmental studies of radioactive particles dust or particulate pollution. Honey is produced by bees as a food source. In cold weather or when fresh food source are scarce, bees use their stored honey as their source of energy. By controlling for bee swarms to nest in artificial hives, people have been able to semi-domesticate the insects and harvest excess honey.

    The bees work together as a group with the regurgitation and digestion until the product reaches a desired quality. It is then store honeycomb cells affer the final regurgitation the honeycomb is le unsealed. However, the nectar is still high in both water content and natural yeast which unchecked would cause the sugars in the nectar to ferment. The process continues as bees inside the hive fan their wings creating a strong across the honeycomb, which enhances evaporation of much of the water from the nectar.

    Honey is collected from wild bee colonies or from domesticated beehives. Wild bee nest are sometime located by following a honey, guide bird. Collecting honey is typically achieved by using smoke from a bee smoker to pacify the bees, this causes the bees to attempt to save resource of the hive from a possible forest fire, and makes than far less aggressive. The honeywomb is removed from the hive and the honey is extracted from that using a honey extractor. Then the honey is laxer filtered. The effect honey has on light is useful for determining the type and quality variations in the water content alter the refractive index of honey. Water content can easily be measured with a refractometer. Typically the refractive index for honey will range from 1.504 at 13% humidity,to 1.474 at 25%. Honey also has an effect on polarized light in that it will rotate the polarization plane. The fructose will give anegative rotation while the glucose will give a positive one. The overall can rotation be used to measure the ratio of the mixture. Moreso, honey has the ability to absorb moisture directly from the air, a phenomenon called hygroscopy. The amount of water the honey will absorb is dependent on the relative humidity of the air. This hygroscopic nature require that honey be stored in a sealed containers to prevent fermentation. Honey will tend to absorb more water in this manner than the individual sugars would allow on their own which may be due to other ingredients it contains.

    Therefore, in Nigeria where the research was carried out, the activities that was conducted is to know the Physicochemical Properties of Honey.


    1.3 Statement of Problems

    Investigation revealed the problems of the Physicochemical Properties of Honey research work which entails that, Honey is replaced with a sugar substitute when it’s removed from the hive. This sugar substitute is not good for bees’ health as it lacks the essential nutrients that honey contains, Selective breeding of honey bees narrows the gene pool and increases the possibility of disease and large-scale die-offs, and Diseases are also caused by importing different species of bees for use in hives (Greenecofriend, 2021).


    1.4 Aim and Objectives of the Study

    The aim of the study is to determine the Physicochemical Properties of Honey. In achieving this aim, the following specific objectives were laid out as follows:

    1. To determine the quality of Nigerian honey in terms of physicochemical properties, and
    2. To verify the compliance of Nigerian honey with international standards.

    1.5 Significance of Study

    This study will be of immense benefit to Agricultural Engineers, Veterinary Doctors, and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.


    1.6 Scope of Study

    The study focuses on the determination of Physicochemical Properties and qualities of Honey in Nigeria


    1.7 Limitations of the Study

    During the course of this study, many things militated against its completion, some of which are:

    1. Time Constraint: The time frame given to accomplish this project was very short due to school academic calendar and it was carried out under pressure which made the researcher not to implement some necessary features.
    2. Research material: availability of research material is a major setback to the scope of the study.
    3. Frequent power failure: This made the researcher append more money on fuel to ensure sustainable power.
    4. Financial Constraint: Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet).

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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    Defense Procedure for Biochemistry Researchers


    In preparation for defending a project or seminar on The Physicochemical Properties of Honey, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


    Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


    Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


    During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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