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Activities of Respiratory Complex Two on the Liver of Normal and Protein Undernourished Rat Fed with Coconut Oil

Activities of Respiratory Complex Two on the Liver of Normal and Protein Undernourished Rat Fed with Coconut Oil

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DEDICATION

This research material, titled “Activities of Respiratory Complex Two on the Liver of Normal and Protein Undernourished Rat Fed with Coconut Oil” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Activities of Respiratory Complex Two on the Liver of Normal and Protein Undernourished Rat Fed with Coconut Oil provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

  • 1.0 Introduction
  • 1.1 Aim of Study

CHAPTER TWO

  • 2.0 Literature Review
  • 2.1 Respiratory complex
  • 2.2 Liver
  • 2.3 Protein Undernourishment
  • 2.4 Coconut oil and its benefits
  • 2.5 Polyunsaturated Fatty Acid
  • 2.5.1 Plasma Triglyceride
  • 2.5.2 Plasma Lipoproteins and Apolipoproteins
  • 2.5.3 Plasma Cholesterol

CHAPTER THREE

  • 3.0 Materials and methods
  • 3.1 Materials
  • 3.1.1 Apparatus and Equipment
  • 3.1.2 Chemicals and reagents
  • 3.2 Experimental animal and diets
  • 3.3 Body weight determination
  • 3.4 Animal Sacrifice
  • 3.5 Preparation of crude homogenate
  • 3.6 Determination of Succinate Dehydrogenase (Complex II)
  • 3.7 Protein determination

CHAPTER FOUR

  • 4.0 Results

CHAPTER FIVE

  • 5.0 Discussion, Conclusion and Recommendation
  • 5.1 Discussion and Conclusion
  • 5.2 Recommendation

REFERENCES


ABSTRACT

In this attempt to investigate the effect the activities of respiratory complex II in the liver of normal and protein undernourished male weaning rats fed with diets containing coconut oil twenty rats were used. They were grouped into four (5 rats in each and fed with normal diets, low protein diets containing 5% casein, normal with coconut oil substituting to vegetable oil and low protein with coconut oil­).

After feeding for six weeks the rats were sacrificed using cervical dislocation. The liver was harvested and homogenized in iced cold buffer and centrifuged at 4000rpm for 15 minutes at 4oC. The pellets were discarded and the supernatant was stored. Further analysis explained later in this work was carried out on this supernatant. The result of this of this analysis showed a significant weight gain in the group fed with normal diet regarded as the control, while in the group fed with low protein diet contain 5% casein had a stunted growth. Thus the group containing coconut oil with the diets in their appropriate proportion experienced a little growth deficiency while a very drastic growth was noticed in the group fed with low protein with coconut oil.


Activities of Respiratory Complex Two on the Liver of Normal and Protein Undernourished Rat Fed with Coconut Oil

CHAPTER ONE

1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.…

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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