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Antibacterial Activity of Honey on Staphylococcus Aureusescherichia Coli and Streptococcus Pyogen Isolated From Wound

Antibacterial Activity of Honey on Staphylococcus Aureusescherichia Coli and Streptococcus Pyogen Isolated From Wound

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DEDICATION

This research material, titled “Antibacterial Activity of Honey on Staphylococcus Aureusescherichia Coli and Streptococcus Pyogen Isolated From Wound” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Public Health for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Antibacterial Activity of Honey on Staphylococcus Aureusescherichia Coli and Streptococcus Pyogen Isolated From Wound provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

  • 1.0 Introduction
  • 1.1 Aims and objectives

CHAPTER TWO

  • 2.0 Literature review
  • 2.1 Wound infection
  • 2.2 Definition of honey
  • 2.3 Local test for honey
  • 2.4 Classification of honey
  • 2.5 Preservation of honey
  • 2.6 Properties and active ingredient of honey
  • 2.7 Mode of action of some antibacterial substance present in honey
  • 2.8 Clinical conditions for treatment with honey
  • 2.9 Honey as an antimicrobial agent
  • 2.10 Practical consideration for the clinical use of honey
  • 2.11 Adverse reaction of honey
  • 2.12 Research on honey

CHAPTER THREE

  • 3.1 Source of Sample
  • 3.2 Sources of honey
  • 3.3 Identification of organisms
  • 3.4 Gram Staining
  • 3.5 Indole test
  • 3.6 Catalase test
  • 3.7 Coagulase test
  • 3.8 Antibacterial sensitivity test

CHAPTER FOUR

  • 4.1 Result

CHAPTER FIVE

  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendation
  • Reference
  • APPENDIX 1
  • APPENDIX 2

ABSTRACT

Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity. The antibacterial activity increased with increase in the concentrations and honey from Nsukka produced a high antibacterial activity (clearer zone) on staphylococcus aureus and Escherichia coli at all concentration and moderately for streptococcus pyogens. The use of honey as a therapeutic substance has been rediscovered by the medical profession on more recent times, and it is gaining acceptance as an antibacterial agent for the treatment of ulcers and bed sores, and other infections resulting from burns and wounds.


Antibacterial Activity of Honey on Staphylococcus Aureusescherichia Coli and Streptococcus Pyogen Isolated From Wound

CHAPTER ONE

1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.…

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.

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