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Antihyperlipidemic and Antioxidant Effects of Phaseolus Vulgaris

Antihyperlipidemic and Antioxidant Effects of Phaseolus Vulgaris

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DEDICATION

This research material, titled “Antihyperlipidemic and Antioxidant Effects of Phaseolus Vulgaris” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Pharmaceutical Technology / Science for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Antihyperlipidemic and Antioxidant Effects of Phaseolus Vulgaris provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



ABSTRACT

In this study, the antihyperlipidemic and antioxidants activities of the extract and fractions ofPhaseolus vulgaris L. were evaluated. The crude extract (PVE) of dried pulverized plant materialwas obtained by maceration in methylene chloride/methanol (1:1) while the solvent fractionswere obtained by successive solvent-solvent partition in separating funnel between the crudeextract suspended in aqueous medium and solvents of increasing polarity to obtain the n-hexanefraction (PVHF), ethylacetate fraction (PVEF), and butanol fraction (PVBF) in thatorder.Antihyperlipidemic effects of the extracts and fractions were investigated using acute, subacuteand chronic models. In all threemodels, treatment with (PVE) caused significantreduction(PSimilarly, very low density lipoprotein (VLDLC)and low density lipoprotein (LDL-C) wasrespectively decreased by 18.18% and 48.73% in theacute antihyperlipidemia model. In contrast, high density lipoprotein (HDL-C) level wassignificantly (PTriglycerides level wassimilarly reduced by 21.59% and 15.91% at PVHF (200 mg/kg) and PVEF (200 mg/kg) with theacute study.The sub-acute protocol showed significant decrease at PVBF 200 mg/kg percentagedecrease of 17.28%. VLDL-C level for the fraction study showed significant decrease (PFinally in the chronic protocol a significant decrease was seen with PVHF 200mg/kg,PVEF 100 mg/kg, and PVBF 200 mg/kg with percentage decrease of 26.03%, 24.82% and20.05% respectively. HDL-C level for extract study showed dose dependent significant increase(PThe highest percentage reduction of DPPH was 80.61% seen with PVEFand PVBF fraction at 400 mg/kg. The highest percentage reduction of nitric oxide was 75.86%seen with PVHF at 200 mg/kg. The in vitro study showed significant increased (P0.05) increase or decrease in the serumMDA of both the treated and untreated group, while in catalase activity estimation showedsignificant (P


Antihyperlipidemic and Antioxidant Effects of Phaseolus Vulgaris

CHAPTER ONE

1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.…

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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