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Sparklyn Effect of Metal Ions on Arginase from the Kidney of Local Goat (Capra Aegagrus Hircus)
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Effect of Metal Ions on Arginase from the Kidney of Local Goat (Capra Aegagrus Hircus)


This page presents an excerpt of the research material, providing a comprehensive overview of the study. It includes the Preliminary Pages, Table of Contents, Abstract, Chapters One to Five, and References, making it accessible and informative for students, researchers, and other readers interested in the topic of this study. Acknowledgement is also included, expressing gratitude to the individuals, institutions, and resources that contributed to the successful completion of the research, with materials and information sourced from the online platform sparklyn.com.ng, which provided valuable academic support.


PRELIMINARY PAGES

  • Title page
  • Approval page
  • Dedication
  • Acknowledgement
  • Table of Contents
  • Abstract

CHAPTER ONE

  • 1.0 Introduction and Literature review
  • 1.1 Introduction
  • 1.2 Literature review
  • 1.2.1 History of arginase
  • 1.3 Occurrence and distribution of arginase
  • 1.3.1 Bacteria
  • 1.3.2 Fungi
  • 1.3.3 Lichens
  • 1.3.4 Yeast
  • 1.3.5 Insects
  • 1.4 The substrate: Arginase
  • 1.5 Scientific classification of local goat
  • 1.6 Aim and Objectives

CHAPTER TWO

  • 2.0 Materials and methods
  • 2.1 Materials
  • 2.1.1 Apparatus used
  • 2.2 Methods
  • 2.2.1 Assay
  • 2.3.2 Protein determination
  • 2.3.3 Purification procedure

CHAPTER THREE

  • 3.0 Results
  • 3.1 Purification and Homogeneity
  • 3.2 Effect of metal ion on arginase from kidney of a local goat

CHAPTER FOUR

  • 5.0 Discussion, Conclusion and Recommendation
  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendations

REFERENCES

.

ABSTRACT


This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.



1.1 Introduction

In this section, Effect of Metal Ions on Arginase from the Kidney of Local Goat (Capra Aegagrus Hircus) is discussed, with relevant and recent citations. As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the aim and objectives of the study. Others are significance of the study, scope of work, research hypothesis and questions, limitation of the study and definition of terms.


CHAPTER TWO

LITERATURE REVIEW


2.1 Introduction

This chapter focuses on the review of related literature. A literature review presents current knowledge, as well as theoretical and methodological contributions, related to Effect of Metal Ions on Arginase from the Kidney of Local Goat (Capra Aegagrus Hircus). It documents the state of the art on the subject under study and provides a comprehensive survey of existing literature. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …


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