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Effect of Metal Ions on Arginase From the Kidney of Local Goat (Capra Aegagrus Hircus)

Effect of Metal Ions on Arginase From the Kidney of Local Goat (Capra Aegagrus Hircus)

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DEDICATION

This research material, titled “Effect of Metal Ions on Arginase From the Kidney of Local Goat (Capra Aegagrus Hircus)” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Effect of Metal Ions on Arginase From the Kidney of Local Goat (Capra Aegagrus Hircus) provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

  • 1.0 Introduction and Literature review
  • 1.1 Introduction
  • 1.2 Literature review
  • 1.2.1 History of arginase
  • 1.3 Occurrence and distribution of arginase
  • 1.3.1 Bacteria
  • 1.3.2 Fungi
  • 1.3.3 Lichens
  • 1.3.4 Yeast
  • 1.3.5 Insects
  • 1.4 The substrate: Arginase
  • 1.5 Scientific classification of local goat
  • 1.6 Aim and Objectives

CHAPTER TWO

  • 2.0 Materials and methods
  • 2.1 Materials
  • 2.1.1 Apparatus used
  • 2.2 Methods
  • 2.2.1 Assay
  • 2.3.2 Protein determination
  • 2.3.3 Purification procedure

CHAPTER THREE

  • 3.0 Results
  • 3.1 Purification and Homogeneity
  • 3.2 Effect of metal ion on arginase from kidney of a local goat

CHAPTER FOUR

  • 5.0 Discussion, Conclusion and Recommendation
  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendations

REFERENCES

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ABSTRACT

This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.


Effect of Metal Ions on Arginase From the Kidney of Local Goat (Capra Aegagrus Hircus)

CHAPTER ONE

1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.…

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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Defense Procedure for Biochemistry Researchers

In preparation for defending a project or seminar on Effect of Metal Ions on Arginase From the Kidney of Local Goat (Capra Aegagrus Hircus), it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.

Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.

Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.

During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.

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