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Estimation of Serum Llipids (Total Cholesterol, HDL Cholesterol and LDL Cholesterol in Alloxan Induced Diabetic Wister Rats)

Estimation of Serum Llipids (Total Cholesterol, HDL Cholesterol and LDL Cholesterol in Alloxan Induced Diabetic Wister Rats)

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DEDICATION

This research material, titled “Estimation of Serum Llipids (Total Cholesterol, HDL Cholesterol and LDL Cholesterol in Alloxan Induced Diabetic Wister Rats)” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Estimation of Serum Llipids (Total Cholesterol, HDL Cholesterol and LDL Cholesterol in Alloxan Induced Diabetic Wister Rats) provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

INTRODUCTION

    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction
    • ⋮

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction
    • ⋮

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • ⋮
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES


    ABSTRACT

    This research work assessed the hypoglycaemic effect of canavaelia ensiforoms and its relationship with serum lipids (total cholesterol, HDL — cholesterol and LDL — cholesterol) in an alloxan induced diabetic rat. Diabetes was induced using 100mg/kg during weight of a 5% stock solution of alloxan. Three were four groups and they were divided into group treated with 200mg/kg body weight of the plant extract, group treated with 400mg/kg body weight of the plant extract, diabetic control group and normal control group. The result after 14 days of treatment with the plant extract showed significant reduction in blood glucose concentration in diabetic rat treated with 200mg/kg of plant extract (94.5 + 25.22mg/dl) as compared to the diabetic control rats (38016 + 133.01mg/dl).

    The effect of the plant extract an serum total cholesterol in diabetic treated rats with 200mg/kg body of (ensiforms also decreased significantly (118.20 + 24.55mg/dl) is compared to the diabetic control rats (201.84 + 61.55mg/dL).

    In addition, there was significant increase in the HDL — cholesterol of the diabetic rat treated with 200mg/kg body weight of the plant extract (57.54 + 16.31mg/dL) as compared to the diabetical rat (3286 + 7.42mg/dL). Finding, there was significant reduction in the LDL — cholesterol of the diabetic rat treated with 200mg/kg body weight of the plant extract (51.59 + 29.82mg/dL) as compared to the diabetic control (133.44 + 57.39mg/dL). Thus, canavalin ensiform extract reduces blood glucose, total cholesterol, LDL — cholesterol and increase HDL — cholesterol.


    Estimation of Serum Llipids (Total Cholesterol, HDL Cholesterol and LDL Cholesterol in Alloxan Induced Diabetic Wister Rats)

    CHAPTER ONE

    1.0 Introduction

    The use of dietary legumes have been reported to influence glycoami and lipiddaemia in man and experimental animals. (Mahnow et l. 1980; Bingwem et al., 1981, Molgaard et l. 1987; Donatucei et al. 1987).

    (anaralis ensiformis DC (family: leguminosaae) variously known as jack beans, though a nature of central America and west idnies has been widely cultivated in the Lumed tropics of Africa and Asia. The seeds have been reported to possess antihypercholesterolaemic (Marfo et al., 1990) and hypoglycemic (enyikwola et al., 1991) activities.

    Diabetics mellitus is a syndrome characterizing by chronic hyperglycemic and disturbances of carbohydrate, fat and protein metabolism associated with absolute or relative deficiencies in insulin secretion and/or insulin action (Bennet, 1994).

    Patients, with this disease are generally classified as either type I (insulin dependent) or type 2 (non — insulin and dependent) diabetics. For oth types of diabetes, control of blood glucose levels, lipids leels,blood pressure and weight will reduce the rise of vascular problems and associated diseases (Williams, 1994).

    Earlier work on the effect of (ensiforms on normal and alloxan induced diabetic rats showed statistically significant decreases in blood and arising urinary glucose weight loss to the treated diabetic animals (Nimen bo — Uadia and Osagie, 1999).

    This study reports the effect of C. ensiforms on total senior cholesterol, HSL — cholesterol and LDL — cholesterol in alloxan — induced diabetic rats.

    …

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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