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Hepatotoxicity of the Ethanolic Extracts of Enantia Chlorantha Root on Wistar Albino Rats

Hepatotoxicity of the Ethanolic Extracts of Enantia Chlorantha Root on Wistar Albino Rats

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DEDICATION

This research material, titled “Hepatotoxicity of the Ethanolic Extracts of Enantia Chlorantha Root on Wistar Albino Rats” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Hepatotoxicity of the Ethanolic Extracts of Enantia Chlorantha Root on Wistar Albino Rats provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE1.0 Introduction

  • 1.1 Background of the Study
  • 1.2 Aims and Objectives
  • 1.3 Statement of Problem
  • 1.4 Limitation of Study
  • 1.5 Significance of the Study

CHAPTER TWO

  • 2.0 Literature Review
  • 2.1 General Description of Enantia Chlorontha
  • 2.2 Ecological Distribution of Enantia Chlorantha
  • 2.3 Management and Propagation Practices
  • 2.4 Functional Uses
  • 2.5 Toxicity
  • 2.6 Hepatotoxicity
  • 2.7 Liver
  • 2.7.1 Liver Enzyme
  • 2.7.2 Some Pattern of Liver Injury
  • 2.7.3 Treatment/Prognosis of Liver Damage
  • 2.8 The Albino Rats
  • 2.9 Phytochemical Compounds

CHAPTER THREE

  • 3.0 Materials and Methods
  • 3.1 Collection, Identification and Processing of Plant Material
  • 3.1.1 Extraction of Plant Material
  • 3.1.2 Determination of the Weight of Dried Extract
  • 3.2 Phytochemcial Analysis
  • 3.3 Experimental Animal
  • 3.4 Determination of Dosage of the Plant Extract For Animal Feeding
  • 3.5 Grouping of Animal and Administration of Plant Extract
  • 3.6 Feeding Method
  • 3.7 Procedures For Blood Sample Collection
  • 3.7.1 Alanine Aminotransferase Test (Alt) Method For Alt Test
  • 3.7.2 Aspartate Animotransferase: (Ast) Method For Ast Test
  • 3.7.3 Alkaline Phousphatase: (Alp) Method For Alp Test

CHAPTER FOUR

  • 4.0 Results
  • 4.1 Result of the Photochemical Analysis of the Ethanolic Extract of Enantia Chlorontha
  • 4.2 Hepatotoxicity Assay

CHAPTER FIVE

  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendation

REFERENCES


ABSTRACT

The root of Enantia chlorontha was assayed for its hepatotoxicity effect. Extacts from the root were made using 95% ethanol. The root extracts were administered orally to 15 wistar albino rats and varying doses. 5 rats were kept as control group. The effect of the extracts was determined by assaying for the levels of serum-liver enzymes acting as biochemical makers. Liver enzymes assayed included Aspartate aminotransferase (AST), Alanin aminiotransferase (ALT) and alkaline phophatase (ALP). Comparison of the enzyme levels of the control group with these of the group administered with the rot extract revealed a significant elevation of liver enzyme levels.


Hepatotoxicity of the Ethanolic Extracts of Enantia Chlorantha Root on Wistar Albino Rats

CHAPTER ONE

1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.…

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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