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Microbiological Examination of Decaying Vegetable (Pumpkin Leaves)

Microbiological Examination of Decaying Vegetable (Pumpkin Leaves)

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DEDICATION

This research material, titled “Microbiological Examination of Decaying Vegetable (Pumpkin Leaves)” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Science Laboratory Technology (SLT) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Microbiological Examination of Decaying Vegetable (Pumpkin Leaves) provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




ABSTRACT

Microbiological Examination of decaying vegetable (pumpkin leave) sold at Afia Nine in Obiagu, Enugu was carried out to obtain the caused organisms, and were examined by culturing them on nutrient agar, MacConkey agar and sabourand dextrose agar for bacteria and fungi organisms that are causing decaying of vegetable (pumpkin leave). Three (3) bacteria and three (3) fungi were isolated. The bacteria include Erwinia carotovora, Pseudomonas solanceatun and Corynebacteria spedonicum while fungi isolated include Aspergilus flavus, Rhizopus and Fusarium graminearum. This research work shows that decaying vegetable (pumpkin leave) were contaminated with pathogenic micro-organisms.



Microbiological Examination of Decaying Vegetable (Pumpkin Leaves)


1.0 Introduction

Pumpkin leaves, fruits and flowers are all protective health giving foods. They contain vitamins and minerals that the body needs to stay healthy. The dark green pumpkin leaves are an excellent source of vitamin A. The body needs vitamin A for proper growth, health eyes and protection from disease. The pumpkin leaves are also a good source of calcium, vitamin C, Iron and protein. Calcium helps to make strong bones and teeth. Vitamin C keeps the body tissue. Iron helps in keeping the body healthy and the body also needs protein to grow and stay healthy. Pumpkin leaves have much more of vitamin A and Vitamin C. pumpkin flowers can also be eaten for it contains more of vitamin A source. The most nutrients of all pumpkin foods are the leaves. They are an excellent source of vitamin A and also vitamin B (Thiamin) and niacin. Vitamin B and niacin helps the body convert carbohydrate into energy and heat (Anee, 2001).

Pumpkins are use for food. The tender leaves and stems, flowers, fruits and seed of pumpkin can all be eaten. Pumpkin leaves can be stored up to six months but can be achieved when they are not touching each other. It is being preserved by drying or freezing. Drying pumpkin must be done carefully in order to kill all their microorganism that would make it spoils. It needs to be dried in hot sun so that it dries quickly before if spoils. Pumpkin leaves also contain a great amount of vitamin A that is vital for a proper growth, healthy eyes and protection form disease. More ever, pumpkin leaves are great source of calcium and they can be prepared in many ways. First of all, leaves should be washed every time before cooking and placed in boiling water (Roberts et al, 2006).

A pumpkin is a gourd like squash of the genus Cucurbita and the family Cucurbitaceae which also include gourds. It commonly refers to cultivars of any one of the species Cucurbita pepo, Cueurbita mitta, Cucurbita mayima and is native to North America. They typically have a thick, orange or yellow shell, creased from the stem to the bottom, containing the seeds and pulp. A pumpkin leaves is an important food for many people. It is a very vulnerable because most parts of the plant can be eaten and are rich in nutrients. Pumpkin is easy to grow and one plant in garden can supply pumpkins and green leaves throughout the year. They grow all round the world for a variety of reasons (George et al, 2008).

Pumpkin belongs to the family with scientific name Cucurbitaceae. They do not need much cooking after once they are planted. They can be grown throughout the pacific even on a toll. Pumpkin plants grow easily from ether the leaves or cutting roots. There are many different varieties of pumpkin which differ in flavour, texture, colour and storing quality. Pumpkin grow beat in loose and rich soil. The site of an old nebbish heap is good place or site for growing pumpkins. Brushing the flowers gently with a feather or brush will help to produce more pumpkin (Susan et al, 1998).

1.1 Statement of Problem

Investigation revealed that bacteria and fungi are associated with the decaying of pumpkin leaves (Sussan, 1998). This statement now prompted the need of this research work.


1.2 Aim and Objectives of Study

The aim of the study is to carry out a Microbiological Examination of Decaying Vegetable (Pumpkin Leaves). In achieving this aim, the following specific objectives were laid out as follows:

  1. To examine decaying pumpkin leaves; and
  2. To ascertain the microbial load that cause decaying in pumpkin leaves.

1.3 Significance of Study

The importance of this project work is to examine the microbial or microbes that are associated with decaying of pumpkin leaves and method of preserving the pumpkin leaves from decaying.


1.4 Limitations of the Study

This project topic is limited to assay bacterial associated with the micro-biological examination of decaying vegetable pumpkin leaves during storage of which the following problem are faced, handicap, time factors, transportation barrier, unconducive laboratory and contamination which could result from laboratory opening such as windows that are always and constantly left unclosed.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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Defense Procedure for Science Laboratory Technology Researchers


In preparation for defending a project or seminar on Microbiological Examination of Decaying Vegetable (Pumpkin Leaves), it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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