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Extraction and Characterization of Orange Seed and Pawpaw Seed Oil

Extraction and Characterization of Orange Seed and Pawpaw Seed Oil

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DEDICATION

This research material, titled “Extraction and Characterization of Orange Seed and Pawpaw Seed Oil” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Science Laboratory Technology (SLT) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Extraction and Characterization of Orange Seed and Pawpaw Seed Oil provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




ABSTRACT

The study determines the Extraction and Characterization process of Orange Seed and Pawpaw Seed Oil. In achieving this aim, the following specific objectives were laid out to determine the nutritive value of orange seed and compare the physicochemical properties of oils extracted from Orange Seed and Pawpaw Seed Oil, investigate the use of the seeds as a source of oil, African Pear and Pawpaw, and expand the knowledge of fatty acid composition of Orange and Pawpaw seed oil, and evaluating its potential application in synthesis of surface coating driers. The seeds of Orange and Pawpaw are generally discarded. However, in order to make a more efficient use of pawpaw and orange, it is worth investigating the use of the seeds as a source of oil. In this work oil was extracted from the seeds of both pawpaw and orange. The specific gravity, iodine value, acid value, peroxide value, saponification value and moisture content of pawpaw seed oil were determined and the result are 0.85, 30.20, 47.12, 48.60, 79.38, and 0.18 respectively. Similarly, the following results were obtained for the orange seed oil; 0.47, 24.6, 51.40, 250.70, 194.24 and 6.43 respectively.



Extraction and Characterization of Orange Seed and Pawpaw Seed Oil


1.1 Introduction

The papaya is the plant Carica papaya, one of the 22 accepted species in the genus Carica of the family Caricaceae. Orange is from the citrus family, the fruit size varies with cultivar and crop load, but most often measures between 2.5-4 inches in diameter. The shape of the fruit is spherical to oblong, with a peel thickness between that of grape fruit and tangerine and is either smooth or roughly pebbly. The presence and amount of seed, depends also on the cultivar (Nwobi et al., 2006).

As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Limitations of the Study and Definition of technical terms.


1.2 Background of Study

The extraction and commercialization of these oils will reasonably enhance the profit status of most fruit juice making industries and encourage the sustenance of the cultivation of the seedy species of sweet orange fruits. The extraction and characterization of oils from fruit seeds have been carried out extensively, but not much data is available on the physio-chemical properties of the oil from the seed of African sweet orange, despite an initial attempt by some authors who worked on the seeds of orange and closely related lime seeds (Nwobi et al., 2006).

The chemical composition of the oil extract consequently gives a qualitative identification of oils and is a very important area in the selective application guide for the commercialization and utility of oil products. Fats and oils are also very important indigenous raw materials for many edibles and non-edible purposes (Abbas Ali et al., 2008).

Orange is from the citrus family, the fruit size varies with cultivar and crop load, but most often measures between 2.5-4 inches in diameter. The shape of the fruit is spherical to oblong, with a peel thickness between that of grape fruit and tangerine and is either smooth or roughly pebbly. The presence and amount of seed, depends also on the cultivar (Nwobi et al., 2006).

However in other to make a more efficient use of papaya and orange, it is worth investigating the use of the seeds as a source of oil. The extraction and use of vegetable oil has for centuries played an important role in the manufacture of large number of industrial product and food items (puangir et al, 2005)

Papaya (Carica papaya L.) is a plant that grows wild in many parts of the tropics. Papaya is important for its fruit and it is cultivated mainly for this purpose (Puangsri et al., 2005). It varies in size, most are oblong, globular or round, often five angled and narrowed at the tip. The inside hollow of the fruit is lined with numerous dark grey seeds. The seeds of papaya and orange fruits are generally discarded. However, in order to make a more efficient use of papaya and orange, it is worth investigating the use of the seeds as a source of oil. The extraction and use of vegetable oils has for centuries played an important role in the manufacture of a large number of industrial products and food items (Puangsri et al., 2005).

Kar and Mital (1999) reported that shea butter is a natural fat obtained from the seeds of the shea tree Butyrespermum parkii. The shea butter fat was extracted from the seeds with various organic solvents, namely petroleum ether, n-hexane, chloroform and benzene. It was concluded that these solvents, particularly petroleum ether and n hexane, could be used for the extraction of shea butter that is free from any oxidized fat and colouring impurities. Akpan et al. (1999) carried out extraction, characterization and modification of castor seed oil and reported that tested parameters which include specific gravity, refractive index, acid value, saponifiation value and iodine value for both crude and refined castor oil produced were of good quality. In fact the iodine value obtained (84.8) for the refined oil indicates that the oil could certainly be used as a lubricant, hydraulic brake fluid and protecting coatings (Kyari, 2008).

Oil yielding crop plants are actually very important for economic growth of agricultural sector. The oilseeds containing unusual fatty acids are very important for industry; they can be used in pharmaceuticals, cosmetics, detergents, soaps, textiles, surfactants (Hosamani and Sattigeri, 2000). On the other hand, no oil from a single source has been found to be suitable for all purposes because oils from different sources generally differ in their composition. This requires the search for new sources of novel oils. Several plants are now growing, not only for food and fodder, but also for a striking range of products with an industrial application (Miladi et al., 2007).

Therefore, in Nigeria where the research was carried out, the activities that was conducted is to know the Extraction and Characterization of Orange Seed and Pawpaw Seed Oil.


1.3 Statement of Problem

Orange seeds, African pear and paw paw seeds are normally discarded and assumed to be a waste product, but the oil from the seed of paw paw, pear and orange has been reported to be useful in pharmaceutical industries, consumerist soaps, textile and detergent industries. These has promoted for a research work to be carried out on the extraction of oil from the seed of paw paw and orange.


1.4 Aim and Objectives of Study

The aim of the study is to determine the Extraction and Characterization process of Orange Seed and Pawpaw Seed Oil. In achieving this aim, the following specific objectives were laid out as follows:

  1. To determine the nutritive value of orange seed and compare the physicochemical properties of oils extracted from Orange Seed and Pawpaw Seed Oil
  2. To investigate the use of the seeds as a source of oil and also diversify the uses of this oils thereby increasing earnings for local farmers as well as encouraging cultivation of Orange and Pawpaw.
  3. To expand the knowledge of fatty acid composition of Orange and Pawpaw seed oil, and evaluating its potential application in synthesis of surface coating driers.

1.5 Significance of Study

This will help to diversify the uses of this oils thereby increasing earnings for local farmers as well as encouraging cultivation of Orange Seed, African Pear and Pawpaw Seed. This study will be of immense benefit to other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.


1.6 Scope of Study

The study focuses on the Extraction and Characterization of Orange Seed and Pawpaw Seed Oil. The orange seeds, African pear and pawpaw seeds were collected from homes within the University of Benin (UNIBEN).


1.7 Limitations of the Study

During the course of this study, many things militated against its completion, some of which are:

  1. Time Constraint: The time frame given to accomplish this project was very short due to school academic calendar and it was carried out under pressure which made the researcher not to implement some necessary features.
  2. Research material: availability of research material is a major setback to the scope of the study.
  3. Frequent power failure: This made the researcher append more money on fuel to ensure sustainable power.
  4. Financial Constraint: Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet).

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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Defense Procedure for Science Laboratory Technology Researchers


In preparation for defending a project or seminar on Extraction and Characterization of Orange Seed and Pawpaw Seed Oil, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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