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The Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase From Human Fibriod Tissue
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The Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase From Human Fibriod Tissue


This page presents an excerpt of the available research material, including the Preliminary Pages, Table of Contents, Abstract, Chapters One to Five, and References. It provides a comprehensive overview of the study, enhancing readability and accessibility for students, and researchers seeking complete material on the topic stated above.


ACKNOWLEDGEMENT


I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Biochemistry for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on The Effect of Sodium Citrate and Reduced Glutathione (GSH) on Arginase From Human Fibriod Tissue provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




PRELIMINARY PAGES


CHAPTER ONE

  • 1.0 Introduction and Literature Review
  • 1.1 Fibroid
  • 1.1.1 Location and classification of fibroid
  • 1.1.2 Pathogenesis and Pathophysiology
  • 1.1.2.1 Pathogenesis
  • 1.1.2.2 Pathophysiology
  • 1.1.3 Signs, Symptoms and Causes of fibroid
  • 1.2 Arginase
  • 1.2.1 Role of arginase in human body
  • 1.2.2 Aginase activities in Benign tumour
  • 1.2.3 Arginase II expression in benign and malignant prostate tissue
  • 1.3 Glutathione (GSH)
  • 1.3.1 Role of glutathione as an antioxidant
  • 1.3.2 Glutathione response to exercise
  • 1.4 Sodium citrate
  • 1.5 Aims and objectives

CHAPTER TWO

  • 2.0 Materials and Methods
  • 2.1 Materials
  • 2.1.1 Apparatus
  • 2.2 Methods
  • 2.2.1 Preparation of buffers and reagents
  • 2.3 Biochemical test
  • 2.3.1 Arginase assay
  • 2.3.2 Protein standard curve
  • 2.3.3 Effect of Glutathione (GSH)

CHAPTER THREE

  • 3.0 Results

CHAPTER FOUR

  • 4.0 Discussion, Conclusion and Recommendation
  • 4.1 Discussion
  • 4.2 Conclusion
  • 4.3 Recommendations

REFERENCES



ABSTRACT


The research is aimed at investigating the effect of sodium citrate and reduced glutathione (GSH) on arginase from human fibriod tissue.

The research shows that arginase enzyme which was partially purified was found to have a specific interaction with its substrate.

The study shows that varying the substrate concentration at constant volume of Sodium citrate and Reduced glutathione (GSH) resulted into an Un-competitive inhibition and Competitive inhibition of enzymes respectively.

The results obtained from this study shows that the inhibition nature of arginase by different inhibitors of enzymes gives a promising approach to abnormal arginase activities in cell proliferation of uterine muscle which leads to fibroid.




1.1 Introduction

… As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the aim and objectives of the study. Others are significance of the study, scope of work, Research hypothesis and questions, limitation of the study and definition of terms.…


CHAPTER TWO

LITERATURE REVIEW


2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …


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