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The Isolation and Identification of Nematode Affecting Tomatoes

The Isolation and Identification of Nematode Affecting Tomatoes

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Reference ID: PS-442-TM

DEDICATION

This research work titled "The Isolation and Identification of Nematode Affecting Tomatoes" is dedicated to God for his enabling grace and to all computer enthusiasts who help to make life a pleasant experience.

ACKNOWLEDGEMENT

I owe my indebtedness to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Zoology, Book Authors and Profound Scholars of existing/related research material for your moral support that facilitated the successful completion of my (Tertiary Institution level). I am grateful to God Almighty and my parent for their financial support in my career. I really appreciate you all for everything, Thank you very much.

ABSTRACT

The study was carried out to isolate and identify the nematode affecting tomatoes. Four (4) sites or area were sampled, using the”simple random sampling technique” to make up the study group namely; Malali, Ungwan Rimi, Stadium round about, Kawo for soil or root-not nematodes from tomatoes plant. Ten (10) plants uprooted randomly at each site(s) visited making a total of forty (40) plants were isolation and identification of nematodes was done at the study area base on movement action, tail, shape and a tutorial book. Result obtained shared occurrence of nematodes as follows; Malali, 342; Ungwan Rimi 40; Stadium Round About, 66; Kawo, 261; making a total of 708 nematodes. Of this number, Crieonemoides accounted for 7(0.990Co); Helicotylenchus 544 (76.840Co); Meloidogyne larva 62 (8.760Co); Pratylenchus 19 (2-680Co); Rotylenchus 64 (9.040Co) and Tylenchus 12 (1.690Co). The researcher; considering the negative effect of these on the yield/production of tomatoes, suggests/profers/and also, when transplanting, intercropping.


The Isolation and Identification of Nematode Affecting Tomatoes

CHAPTER ONE

1.0 Introduction

Nematodes are tiny, thread-like worms measuring 0.0 15 inch to 0.187 inch in length. They are either free living parasitic or saprophytic, identified on the basis of shapes, size and special structures. The females become swollen and flask-shaped as a result of accumulation of eggs with the anus virtually terminal in position, while the males are vermiform (Sherf and Macnah, 1986; Chitwood, 1949; Taylor and Sasser, 1978; Idowu, 1979 and Idowu, 1983). Nematodes are known for causing destructive diseases of crops as they have a wide range of feeding habit, constitute about 80% of all multicellular animals, attacking nearly every crop that is grown in the field and as a result crop yields is greatly affected reducing quantity and quality of crops on field, orchard, home garden and green houses (Mai, 1985; Symth, 1994; Sasser, 1952). Among the favoured host in Nigeria as a whole include tomato, yam, tobacco, papaw, citrus and sweet potato (Sasser, 1954).

1.1 Tomato

Tomato (Lycopersicum esculentum) belongs to the family Solanaceae and subilass polypetalae of the dicotyledenous group of plants. Tomato is a slight modification of tomato the name used by the Indians of Mexico, who have grown the plant for food since prehistoric times. Other names reported by early European explorers were tomato, tumatle and tomatas, probably variants of Indian words (Wener, 2004).


1.2 Origin

The precise origin of tomato remains a mystery but there is reason to believe that the original tomato came from Peru called tomato, it was taken to Mexico by migrating Peruvians. It found its way to Italy through the explorations of Christopher Columbus. Tomatoes were taken back to Europe along with silver and gold and they were grown on the continent as a pretty curiosity (Fallagatter, 1999). Though, tomato has become one of the most popular and widely grown vegetables in the world (Chung, 1998), until the 19th century, it was grown chiefly as an ornamental plant for its colourful fruit (Villareal, 1980). This is because it was regarded with suspicion due to the reputation of Solanum-like fruits being poisonous (Philips and Rix, 1993).


1.3 Study Area

Kaduna is the State capital of Kaduna State in north central Nigeria. The city, located on the Kaduna River, is a trade center and a major transportation hub for the surrounding agricultural areas with its rail and road junction.

The Kaduna River is a tributary of the Niger River which flows for 550 kilometers through Nigeria. It got its name from the crocodiles that lived in the river and surrounding area. Activities going on there includes; fishing, packing of sand and farming such as maize, tomato, spinach, cocoa-yam, okro, pepper etc.


1.4 Purpose Of The Study

The destruction of plants comes from organisms including nematode and insect pest. However, this study is restricted only to those plant nematodes that affect tomatoes grown in some selected area of River Kaduna. Many plants are damage by plant parasitic nematodes which feeds and multiply in or on root, stem spreading soil borne viruses or facilitate secondary infection by bacteria and algae.

All kind of living organisms are dependent on plants in one way or the other for their food supply. Therefore, controlling nematode that destroy our crops, it is almost important both agriculturally, economically and socially for well being of both living organism. Although important contributions in nematology are coming in from many countries. Like Nigeria, generally expanded it is in view of hope that it help in one way or the other in preventing or controlling plants nematodes, as the knowledge of the nematodes themselves is important toward successful nematode control.


1.5 Aims and Objectives of Study

The aim of the study is to isolate and identify the nematode affecting tomatoes. In achieving this aim, the following specific objectives were laid out as follows:

  1. To determine the distribution or assessment of nematode populations
  2. To isolate and identify nematode; and
  3. To suggest proper suggestion on minimizing infection

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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