Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria

Garlic (Allium sativum) is a medicinal plant mainly used as condiments and for stopping in different cooking. The use of higher plants and their extracts to treat infections is an ancient practice in traditional medicine. The study evaluates the Antibacterial Activity of Garlic Extract against some Urinary tract Pathogenic Bacteria. In achieving this aim, the following specific objectives were laid out as follows to: identify the activity of garlic extracts on both Gram negative and Gram positive standard bacterial strains and compares their effect with the effect of some antibiotics and assess the anti-bacterial effect of garlic against standard isolates of S. aureus and E. coli. Four different solvents having different polarity were used to extract the bioactive compound from garlic. The Antibacterial activity of the crude extracts of garlic was evaluated against Standard isolates of S. aureus and E. coli by an agar diffusion method. The trial was done in triplicates. A Factorial Design with three factors was used. The treatment means were compared by a Student’s t- test with least significant difference (LSD) at 5% (P=0.05) and the data analysis was performed using mini tab statistical software package. In this experiment the non-polar chloroform had higher inhibition zone. The highest yield potential was obtained from water followed by ethanol, chloroform and petroleum ether respectively. E. coli were so susceptible than S. aureus to the extracts. Garlic could be used as effective antibacterial agent for human pathogenic bacteria. This study will be of immense benefit to Microbiologist, Biochemist and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work.