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Isolation and Characterization of Microorganisms Associated with Diesel Polluted Environment

Isolation and Characterization of Microorganisms Associated with Diesel Polluted Environment

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DEDICATION

This research material, titled “Isolation and Characterization of Microorganisms Associated with Diesel Polluted Environment” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Science Laboratory Technology (SLT) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Isolation and Characterization of Microorganisms Associated with Diesel Polluted Environment provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




PRELIMINARY PAGES


CHAPTER ONE

  • 1.0 Introduction
  • 1.1 Objectives of Study

CHAPTER TWO

  • 2.0 Literature Review
  • 2.1 Environmental effects of oil Pollution
  • 2.1.1 Effect of oil pollution on the marine environment
  • 2.2 Oil Biodegradation
  • 2.3 Microbial Biodegradation
  • 2.4 Fungal Biodegradation
  • 2.5 Types of Oil Consuming Bacteria
  • 2.6 Aerobic Biodegradation of Oil
  • 2.7 Anaerobic Biodegradation of Oil
  • 2.8 Prevention of Oil Spillage

CHAPTER THREE

  • 3.0 Materials and Methods
  • 3.1 Sample Collection
  • 3.2 Sample Analysis
  • 3.3 Identification of Fungal Isolates
  • 3.4 Identification of Bacterial Isolates
  • 3.4.1 Gram staining
  • 3.4.2 Spore Staining
  • 3.5 Other Biochemical Test

CHAPTER FOUR

  • 4.0 Results
  • 4.2 Discussion
  • 5.0 Conclusion
  • 5.2 Contribution to Knowledge

REFERENCES

APPENDIX



ABSTRACT

The bacterial and fungal isolates of a diesel polluted soil in comparison to a control soil were enumerated and identified. Results obtained indicates that the control soil had higher total heterotrophic bacterial (THB) and fungal (THF) than the polluted soil, while the polluted soil had higher hydrocarbon utilizing bacterial (HUB) and fungal (HUF) than the control.

The microorganism isolated included Pseudomonas, Bacillus, Micrococcus, Staphylococcus, Streptococcus, Penicillum, Fusarium, Aspergillus Mucor and Rhizopus species. This study indicates that diesel polluted soil are not suitable for agricultural purposes unless recovery is encourage through treatment.



Isolation and Characterization of Microorganisms Associated with Diesel Polluted Environment


1.0 Introduction

Environmental pollution is a cause of major concern affecting ecosystem globally. Diesel pollution is the release of a liquid petroleum hydrocarbon into the environment due to human activity and is a form of pollution release of effluents and offshore drilling activities is adversely affecting the environment (Atlas, 1981). Oil can also be released into the environment from natural geologic seeps on the sea floor, some times killing area flora and fauna. Oil is the most common pollutant in the environment.

The Niger Delta area of Nigeria Supports a lot of petroleum exploration and exploitation activities, which had subsequently led to a wide scale pollution of its rivers, swamps and farmlands with hydrocarbon (Odokuma, et al 1992). The massive and extensive pollution of these environments constitute socioeconomic and public health hazards (kobayashi et al 1982).

According to Rowell and Florence (1993) Sulphate concentrations are found to be greater in soils disturbed by oil drilling, while dehydrogenase and arylsulphatase activities are lower. Chikere and Okpokwasili (2001) pointed out that the result of these additions in an inhibition of the growth of some autochthonous flora and fauna while encouraging some others to grow, thereby destroying the biodiversity and productivity of such ecosystems.

Oil pollution in the sea or ocean has steadily increases and these cause damage to aquaculture resources by physical contamination, toxic effects and by disrupting business activities. The oil penetrate up the environment from natural oil seep. Most human made oil pollution comes from land-based activity, but public sharply on seagoing oil tankers (Michael Hogan, 2008). Inappropriate disposal of residue generated in different industrial petroleum processes has been a great concern because of the intensity of the environmental damage they can provoke.

Environmental pollution defined as the unfavorable alteration of the surroundings through direct or indirect effects of changes in energy patterns, radiation levels, chemical and physical constitution and abundance of organisms is clearly reflected in the Niger Delta. The study of microorganism in relation to the environment where they are found is becoming very important since they have been found to be minute alterations around them. It is also a certainty that they adapt easily to most of these changes quite unlike most other organisms.

They effectiveness of microorganisms as agent in the destruction and recycling of organic matter in the biosphere is widely acknowledged. Microorganisms are considered to be efficient biodegraders due to their abundance (Regis 2001). Oil pollution can be categorized, ranging from very light to very heavy oils. Most oils are less dense than water and tends to float as a thing layer on the surface water. Once oil gets in the environment, it undergoes weathering which is the process that describes the physical, chemical and biological changes that occurs when oil interact with the environment.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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