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Isolation and Identification of Bacteria Associated with (Tooth Ache) Dental Caries and Bleeding Gum and the Effect of Some Tooth Pastes

Isolation and Identification of Bacteria Associated with (Tooth Ache) Dental Caries and Bleeding Gum and the Effect of Some Tooth Pastes

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DEDICATION

This research material, titled “Isolation and Identification of Bacteria Associated with (Tooth Ache) Dental Caries and Bleeding Gum and the Effect of Some Tooth Pastes” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Science Laboratory Technology (SLT) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Isolation and Identification of Bacteria Associated with (Tooth Ache) Dental Caries and Bleeding Gum and the Effect of Some Tooth Pastes provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




ABSTRACT

Isolation and identification of bacteria associated with dental caries (Tooth ache) and bleeding gum and the effect of some tooth pastes (Macleans) was evaluated using a mouth swap collected from a patient at dental school Trans Ekulu Enugu, and three tooth pastes collected from Ogbete main market Enugu. The three tooth pastes are close up, Dabur herbal tooth past and oral B. The mouth swap was cultured using Nutrient medium and MacConkey medium. The bacteria organisms isolated were Streptococcus mutan and Lactobacillus spp.

The chief dominant bacteria causing dental caries and bleeding gum isStreptococcus mutan. based on the analysis the inhibitory effect of oral B is the highest since it have up to 7.omm clear zone followed by Dabur herbur tooth paste which also have clear zone of about 4.2mm. Close up also have effect on tooth ache since it have about 3.0 clear zone. Therefore the use of these three tooth pastes can reduce or core dental caries (tooth ache) and bleeding gum.



Isolation and Identification of Bacteria Associated with (Tooth Ache) Dental Caries and Bleeding Gum and the Effect of Some Tooth Pastes


1.0 Introduction

1.1 Background of the Study

Tooth ache (dental caries) usually refers to pains around the teeth primarily occur as a result of a dental condition. Tooth ache is mostly caused by tooth problems like; dental cavity, a cracked tooth, an exposed root or gum disease. Dental caries (also known as tooth decay or cavity) results in tooth ache. It is an infection of bacterial origin that causes demineralization and destruction of hard tissue (enamel, dentin and cementum), usually by production of acid by bacterial fermentation of the food debris accumulated on the tooth surface. If this remineralization exceeds, saliva and other demineralization factors such as from calcium and fluoridated tooth pastes, these hard tissues progressively break down, producing dental caries ie cavities, holes in the teeth.

The bacteria most responsible for dental cavities are the mutants streptococci most prominently Streptococcus Mutans, Streptococcus Sobrinusand Lactobacilli. If left untreated, the infection or disease can lead to tooth pain, tooth loss and infection.

John et al (2009), Bleeding gum generally indicates gum disease or risk of gum. This occurs when gums get swollen and tender either due to infection or irritation, they can form bleed plague buildup on gum causing bacteria to grow in the gum. This leads to gingivitis or inflammation of the gums. Gums can also bleed due to imitation caused by chewing certain foods, improper brusting, Hormonal changes during pregnancy, improperly fitted dentures, thyroid disorders, Leukemia and bleeding disorder.


1.2 Aim and Objective

  1. To know or determine the bacterial organisms associated with toot ache (dental caries) and bleeding gum.
  2. To be able to isolate those bacterial organisms that causes bleeding gum and dental caries.
  3. To know the effect of some tooth pastes especially (maclean on the tooth problems.
  4. To educate people (both young and old) on how to take proper care of their teeth and maintain a good oral hygiene and make a wise choice of the type of food to go for so as to maintain a healthy teeth.

1.3 Statement of Problem

The high rate of dental damages and bleeding gum among people of all ages. These damages have caused a lot of loss of teeth, eruption of the affected teeth and even change of colour of teeth among the young beautiful and handsome youths and old adults. Many old people in the vikages cannot comfortable chew hard, hot, and cold foods because of dental problems. Some young people whose teeth have a bad look cannot boldly open their moth publicly and talk. Yet the cause of the high rate of these dental damages was not clearly understood and hence, the topic. To determine and isolate those bacteria that are capable of causing dental problems (tooth ache), particularly dental caries and bleeding gum.


1.4 Significance of the Study

This work is highly beneficial to those who suffer from dental caries (tooth ache) and bleeding gum by providing a guild on how to control the disease and stop further spread and damages. Those who are at risk of getting this diseases because of their choice of food and poor oral hygiene, thereby exposing their teeth to bacterial contamination. They are educated to reduce the frequency of their surgar intake as this is the cause of dental problems foods like vegetables, cheeses, milks, yogurt etc are preferable. The world at risk can also maintain proper oral hygiene by brushing and flushing their teeth every morning and at night before bedtime and possible after every meal. People who just pick any tooth paste without considering its composition or content can now look for those tooth pastes that have fluoride content and those produced from natural herb extracts.

The knowledge of the type of micro-organisms associated with dental caries and bleeding gum will help to find out adequate drugs that can quire the diseases.


1.6 Limitations of the Study

There is lack of adequate financial support which resulted in some difficulties in carrying out the practical, such problems are buying of materials needed for carrying out the practical, transportation the Laboratory where the practical is being carried out and provision.

Also during the collection of the sample, some micro- organization in the mouth and those around the teeth were introduced in the sample and as the suspected micro- organisms were growing these surrounding micro- organisms also grew and there was difficulty in differentiating between them.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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Defense Procedure for Science Laboratory Technology Researchers


In preparation for defending a project or seminar on Isolation and Identification of Bacteria Associated with (Tooth Ache) Dental Caries and Bleeding Gum and the Effect of Some Tooth Pastes, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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