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Isolation and Identification of Microorganism in Stream Water

Isolation and Identification of Microorganism in Stream Water

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DEDICATION

This research material, titled “Isolation and Identification of Microorganism in Stream Water” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Microbiology for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Isolation and Identification of Microorganism in Stream Water provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




PRELIMINARY PAGES


CHAPTER ONE

INTRODUCTION


    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES



    Isolation and Identification of Microorganism in Stream Water


    1.0 Introduction

    Water is the one of the most common substances known and it is good solvent for many substances, water occur at room temperature as clear, colourless, tasteless liquid which freezing into ice at zero degree centigrade and boil at 1000c. it is essential for life on earth.

    According to World Health Organization (WHO) in 1967 is the most important need of living things, it is second only to the air we breath, in fact some scientist believe that all life began in water. Also water is a molecule that consist of two hydrogen atoms and one oxygen atom, when these atoms are heated to a high temperature they join to for water. It occurs in three natural states solid, liquid and gaseous states. Water and ice covered about 75% of the earth surface and water vapour is an important constituent of the atmosphere (Ababio 1985) It exists under major two types which include:.


    Surface Water:

    They include the streams, lakes, and shallow wells. The air through which the rain passes may contaminate the water.


    Ground Water:

    They originate from deep well and subterraneous springs. This is virtually free of bacteria due to filtering action of soil deep sand and rock. However, it may be come contaminated when it flows along the channels.

    Water in Microbiology can be contaminated when it contains a chemical or biological poison or an infectious agent. These condition also apply to water which is polluted except that the agent or poison is often obvious and water carries an unpleasant taste or appearance. (Encyclopedia America 1988).

    Portability refers to the drinkability of water when it is fit for drinking, when unportable it is unfit for consumption due to some contaminant or pollutant (Earnet 1974) other sources of contamination are various types of establishments and agricultural farm etc. Possible sources of microbial contamination of a body of water are soil and agricultural run-off, farm animals, industrial waste, discharges from sewage treatment plants and storm water run-off from urban areas.

    Contamination by sewage or human excrement and pollution by animals causes the greatest danger associated with drinking water in most developing countries. So it is necessary to examine the quality of water before consumption.
    Monitoring and detection of indicator and disease causing microorganisms are a major part of sanitary microbiology. Bacteria from the intestinal tract generally do not survive in the aquatic environment. They are under physiological stress and gradually lose their ability to form colonies on differential and selective media (Prescott 7th Edition).


    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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    Defense Procedure for Microbiology Researchers


    In preparation for defending a project or seminar on Isolation and Identification of Microorganism in Stream Water, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


    Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


    Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


    During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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