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Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of a Cattle

Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of a Cattle

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Reference ID: PS-24317-TM

DEDICATION

This research work titled "Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of a Cattle" is dedicated to God for his enabling grace and to all computer enthusiasts who help to make life a pleasant experience.

ACKNOWLEDGEMENT

I owe my indebtedness to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Animal Science, Book Authors and Profound Scholars of existing/related research material for your moral support that facilitated the successful completion of my (Tertiary Institution level). I am grateful to God Almighty and my parent for their financial support in my career. I really appreciate you all for everything, Thank you very much.


Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of a Cattle

CHAPTER ONE

1.1 Introduction

Cattle are large bodied ruminants that feed on pastures and forages or fodder. In Nigeria, cattle are reared primarily for meat which is a veritable source of protein for humans, and for milk (Arowolo et al., 2013). Cattle rearing in Nigeria is an old occupation which is traditionally practiced by Fulanis and Shuwa Arabs in northern Nigeria with an extremely few local villages in the southern Nigeria (Erebor, 2003).

As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitations of the Study and Definition of technical terms.


1.2 Background of Study

Staphylococcus aureus is a facultative anaerobic gram-positive coccal bacterium and due to a combination of numerous bacteria immune-evasive strategies which it uses, it is considered a successful pathogen. The nasal passages is considered to be the major habitat (Kluytmans et al., 1997; Lowy, 1998; Lowy, 2003) and the biggest supply of S. aureus in people, yet numerous body locales can harbor this bacterium (Vandenbergh & Verbrugh, 1999). S. aureus is a typical tenant of the skin (Lowy, 2003; Williams, 1963), perineum and can likewise be found in the axillae (Ridley, 1959), vagina (Guinan et al., 1982) and the gastrointestinal tract (Williams, 1963). S. aureus strains are noteworthy human pathogens and are conceivably ready in contaminating any human body tissue, bringing on everything from skin contaminations to life-debilitating sicknesses. In people, the diseases brought on by S. aureus can be partitioned into these three sorts in general; shallow sores, (for example, surgical site and wound contaminations), life and systemic undermining factors, (for example, osteomyelitis, endocarditis, pneumonia, mind abscesses/wounds, bacteraemia and meningitis), then toxinoses, (for example, poisonous stun disorder, sustenance harming and singed skin disorder (Alo et al., 2013; Aires de Sousa et al., 2004; Lowy, 2003). The sign of staphylococcal contamination are the boils that contain discharge which is made up of dead neutrophils, dead and living microbes, tissue (necrotic), the lysed host substance and bacterial cells. The immunocompetent hosts, as a rule, effectively clear the disease and deplete the ulcer, though for the immunocompromised and sporadically for a sound individual, the contamination might advance to a more profound tissues and turn into a conceivably lethal intrusive contamination (Norvick, 2006). It is still one of the five most common causes of nososcomial infections, often causing postsurgical wound infections (Bowersox, 1999). S. aureus is likewise known to colonize and contaminate both pets and animals, including pooches, felines, rabbits, stallions, steers and pigs (Morgan, 2008). A noteworthy concern is the nearness of methicillin safe S. aureus (MRSA) in pets and domesticated animals, as these may fill in as repositories for human colonization, an illustration is MRSA ST398 from pigs (Weese, 2010).

The unnecessary utilization of antibiotics has prompted to the rise of different medication safe strains of S.aureus (Lowy, 1998). The Penicillin was presented for curing infections caused by S. aureus in the 1940s, and adequately diminished mortality and bleakness. Be that as it may, in late 1940s, its resistance because of the nearness of penicillinase developed (Eickhoff, 1972). The staphylococci are extremely fit for advancing imperviousness to the regularly utilized antimicrobials, for example, erythromycin (Walmark & Finland, 1961), ampicillin (Klein and Finland, 1963), and antibiotic medication (Eickhoff, 1972). Much of the time, imperviousness to antimicrobial agents is coded for by qualities carried on plasmids, representing the quick spread of resistant microscopic organisms (Morris et al., 1998). One purpose behind the proceeding with essential part of S. aureus in illness is its inclination and propensity to wind up distinctly impervious to antimicrobial (Waldvogel, 2000). S. aureus is presently the main general reason for nosocomial diseases and, as more patients are dealt with outside the healing center setting, is an expanding worry in the group (CDC NNIS System, 2001; Diekema, 2001).

The time of medication development and its execution in human and creature wellbeing and horticulture was started by the revelation of anti-infection agents over 70 years prior. These disclosures were powerful against organisms consequently were viewed as effective against pathogenic microorganisms however this achievement was fleeting as they were tempered with in all cases by the rise of resistant microorganisms (D'Costa et al., 2011). A standout amongst the most relentless issues confronted by human services benefits far and wide is the expanding pervasiveness of antimicrobial resistance. This resistance is broadly perceived as a noteworthy general wellbeing danger and this issue is aggravated by a consistent reducing of the quantity of new specialists (antimicrobials) entering the clinical practice (D.H, 2000). There is an expanding worry that some less-alarming infections which were effortlessly treated are currently turning out to be progressively hard to treat and ailments created by microscopic organisms which are impervious to antimicrobial agents may set aside a more drawn out time of opportunity to treat successfully (Butler et al., 2006). In spite of the fact that the issue of multidrug resistance has pulled in the consideration of medicinal services administrations and the overall population, rates of antimicrobial resistance among healing center and group pathogens have expanded alarmingly amid the previous decade (NNIS, 2001).

Therefore, in Nigeria where the research was carried out, the activities that was conducted is to know the Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of Cattle.


1.3 Statement of Problems

In Nigeria, cattle are reared primarily as a source of meat. According to Kuehnert et al. (2006), Lowy (1998, 2003), Onanuga & Temedie (2011), Vandenbergh & Verbrugh (1999), Williams (1963), the nares and the skin of humans and animals may be considered as ecological niche for S. aureus colonization but this colonization does not frequently result into infection thereby tagging the bacterium a normal flora of these body parts. S. aureus colonizes the nares and the skin but if there is an abrasion, lesion or wound in these parts, S. aureus may migrate into the body or blood and cause infections. These infections are called opportunistic (staphylococcal) infections. Compared to other pathogens, S. aureus has a high tendency and proneness to become resistant to antimicrobials (Weese, 2010). This fact, coupled with the constant abuse of drugs and lack of control in the sales of antibiotics contributes to the increasing problem in multidrug resistance of S. aureus including methicillin and vancomycin which is considered as the first line of treatment against methicillin resistant Staphylococcus aureus (MRSA).

Cuny et al. (2015), Fluit (2012), Johnson (2011) and Morgan (2008) established that methicillin resistant S. aureus (a multidrug resistant organism) may not only be a nosocomial and community acquired infection but it could also be a zoonotic infection as it can be transmitted from animal to human. The presence of multidrug resistant S. aureus in the nares of cattle poses a threat to cattle herders, butchers, beef retailers/handlers and consumers as these cattle are frequently asymptomatic carriers hence are considered ‘healthy’. This study may evaluate the prevalence of multiple antibiotic resistant livestock associated S. aureus and suggest possible control to diseases caused by multiple antibiotic resistant livestock associated S. aureus.


1.4 Aim and Objectives of Study

The aim of the study is to evaluate the Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of Cattle. In achieving this aim, the following specific objectives were laid out as follows:

  1. To isolate and identify S. aureus from nasal passage of healthy cattle by Gram staining and biochemical tests (catalase test, slide coagulase test and fermentation of mannitol);
  2. To determine the antibiogram of the isolates and calculate the Multiple Antibiotic Resistance Index (M.A.R.I) using the antibiogram of the isolates;
  3. To determine the minimum inhibitory concentration (μg/disc) of the S. aureus isolates to vancomycin;
  4. To determine the minimum inhibitory concentration (μg/ml) of the isolates to flucloxacillin as a test for methicillin resistant S. aureus (MRSA) and vancomycin and
  5. To determine the synergistic, antagonistic or additive effect of two antibiotics to suggest possible control of staphylococcal infections in human caused by multidrug resistant livestock associated S. aureus.

1.5 Research Questions

The study came up with research questions so as to be able to ascertain the above stated objectives. The specific research questions for the study are stated below as follows:

  • Can S. aureus be isolated and identified from nasal passage of healthy cattle?
  • Are the effects of the combined antibiotics synergistic, additive or antagonistic?
  • How is the antibibiogram of S. aureus isolates determined and how are the Multiple Antibiotic Resistance Index of the isolates calculated using the antibiogram of the isolates?
  • How is the minimum inhibitory concentration (μg/disc) of S. aureus determined?
  • How is the minimum inhibitory concentration (μg/ml) of S. aureus isolated from nasal passage of healthy cattle to flucloxacillin and vancomycin determined?

1.6 Research Hypothesis

In order to pursue the objective of this study, the following generalized statements have been designed to guide and aids in obtaining the result for the experiment to be conducted. For this work, the null hypothesis will be represented with H0 while the alternative hypothesis will be represented with hypothesis H1.

  • H0: There are no significant effects of the combined antibiotics synergistic, additive or antagonistic
  • H1: There are significant effects of the combined antibiotics synergistic, additive or antagonistic

1.7 Significance of Study

The following are the relevance of the research work;

  1. The result of the study may create more awareness on the danger of multidrug resistant S. aureus in ruminant flocks.
  2. It will educate the general public on the effect of negligence of multidrug resistant S. aureus in cattle and its effect in the consumption of undercooked beef.
  3. The result may suggest possible control of staphylococcal infections in humans caused by livestock associated S. aureus.
  4. The study may provide a more recent data on multiple antibiotic resistant S. aureus and suggest possible control of livestock associated staphylococcal infections in human.

This study will be of immense benefit to other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.


1.8 Scope of Study

The study focuses on the Prevalence of Multi Drug Resistant Bacteria Isolated for Nasal Passage of Cattle in Nigeria.


1.9 Limitations of the Study

During the course of this study, many things militated against its completion, some of which are:

  1. Time Constraint: The time frame given to accomplish this project was very short due to school academic calendar and it was carried out under pressure which made the researcher not to implement some necessary features.
  2. Research material: availability of research material is a major setback to the scope of the study.
  3. Frequent power failure: This made the researcher append more money on fuel to ensure sustainable power.
  4. Financial Constraint: Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet, questionnaire and interview).

1.10 Definition of Terms

E.coli: Is an acronym for Escherichia coli which is a bacterium commonly found in the intestines of humans and other animals, some strains of which can cause severe food poisoning.

Staphylococcal: Staphylococcus (staph) is a group of bacteria. There are more than 30 types. A type called Staphylococcus aureus causes most infections. Staph bacteria can cause many different types of infections, including; skin infections which are the most common types of staph infections.

Isolates: isolate simply means cause (a person or place) to be or remain alone or apart from others.

Phytochemical: This is a substance produced mainly by plants, and these substances have biological activity.

Component: it simply means one part of a whole thing.

Antibacterial: Anything that destroys bacteria or suppresses their growth or their ability to reproduce. Heat, chemicals such as chlorine, and antibiotic drugs all have antibacterial properties.

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

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