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Screening of Cellulolytic Microorganism from Dumping Sites

Screening of Cellulolytic Microorganism from Dumping Sites

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DEDICATION

This research material, titled “Screening of Cellulolytic Microorganism from Dumping Sites” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.



ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Microbiology for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Screening of Cellulolytic Microorganism from Dumping Sites provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.



TABLE OF CONTENTS

PRELIMINARY PAGES

CHAPTER ONE

INTRODUCTION

    CHAPTER TWO

    LITERATURE REVIEW

    • 2.1 Introduction
    • ⋮

    CHAPTER THREE

    MATERIALS AND METHODS

    • 3.1 Introduction
    • ⋮

    CHAPTER FOUR

    RESULTS AND DISCUSSION

    • 4.1 Introduction
    • 4.2 Results
    • ⋮
    • 4.3 Discussion of Findings

    CHAPTER FIVE

    SUMMARY, CONCLUSION AND RECOMMENDATION

    • 5.1 Introduction
    • 5.2 Summary
    • 5.3 Conclusion
    • 5.4 Recommendation

    REFERENCES


    ABSTRACT

    The study screens the Cellulolytic Microorganism from Dumping Sites using Imo State dumping site as a case study. In achieving this aim, the following specific objectives were laid out to identify the Microorganism Isolated from Dumping Sites, isolate the Cellulolytic bacteria from Waste Dumping Sites and screen and characterize cellulolytic bacterial isolates. Twenty five biodegradable waste samples were collected from different waste dumping sites of Owerri district from five locations viz., Nekede, Obinze, Naze, Irere, and Ihiagwa. The cellulolytic bacteria were isolated on Carboxyl Methyl Cellulose Agar Medium (CMC) by following serial dilution pour plate method. The cellulolytic bacterial isolates were screened qualitatively as well as quantitatively at three different temperatures (10, 15 & 20oC) and three different pHs (5, 7 and 9). Out of twenty, CBW3 isolate from Wadura showed highest solubilization zone (4 mm) with solubilization efficiency (285.7%) and cellulase activity (2.917 U/ml) followed by CBB3 from Baramulla town (3.4 mm) with solubilization efficiency (261.5%) and cellulase activity (2.566 U/ml). All the 20 cellulolytic bacterial isolates were morphologically, biochemically characterized. Although these microbes showed the cellulose activity under variable pH and temperature combinations but pH 7 and 20 oC temperature was the most ideal standardized condition for the better performance by the microbes under investigation. Microorganisms are useful in producing foods, treating waste water, creating biofuels and a wide range of chemicals and enzymes. They are invaluable in research as model organisms. They have been weaponries and sometimes used in warfare and bioterrorism. They are vital to agriculture through their roles in maintaining soil fertility and in decomposing organic matter.


    Screening of Cellulolytic Microorganism from Dumping Sites

    CHAPTER ONE

    1.1 Introduction

    … As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitation of the study and Definition of technical terms.…

    CHAPTER TWO

    2.0 Literature Review

    2.1 Introduction

    This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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