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Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital

Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital

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DEDICATION

This research material, titled “Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Science Laboratory Technology (SLT) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




ABSTRACT

This work was carried out to investigate the antimicrobial activities of Eupatorium odoratum extracts on Pseudomonas spp isolated from wound infections. The plant extract was made using ethanol, cold water and hot water. Various dilutions of the extract were prepared in a distilled water, 50µm/ml, 25µm/ml, 12.5µm/ml, 6.25µm/ml, and 3.125µm/ml. The results obtained showed that the plant extract in ethanol had a higher medicinal value with the minimum inhibition concentration (MIC) of 6.25µm/ml dilution inhibiting the growth of the test organism.

This was followed by the plant extract in hot water which inhibit the organism at the minimum inhibition concentration (MIC) of 6.25µm/ml dilution. The plant extract in cold water had the least medicinal value with the minimum inhibition concentration (MIC) of 12.5µm/ml dilution inhibiting the growth of the test organism (Pseudomonas spp). The research shows that the active ingredients of the plant are better extracted with ethanol than other solvents and it shows that the extracts of the leaves ofEupatorium odoratum at low concentration inhibits the growth ofPseudomonas spp.



Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital


1.0 Introduction

1.1 Background of the Study

Eupatorium odoratum (family Asteraceae) is a Christmas bush, also known as bitter bush, Siam weed, baby tea, Satan Maria, is a scrambling shrub. It is a perennial shrub, exotic to Nepal, due to its adverse growth, it is also called Banmara. Stems reach 2cm in diameter. The plants are maintained by a system of abundant, yellowish fine lateral roots. Multiple sprouts arise from the root crown and lower stems (Irobi, 2004). The individual branches are long with relatively few branches. The opposite, three nerved leaves are deltoid to ovate — lanciolate, usually with a dentate margin and a long pointed tip. The leaves are aromatic when crushed. The inflorescences are corymbs of cylindrical heads located on the terminals of lateral branches. There are 15 to 25 tubular florets per head, white, lavender, pink, or blue in colour. The seeds are brownish gray to black achene that is 4mm long with a pale brown pappus 5 or 6mm long (Iwu, 2005).

Eupatorium odoratum is an aromatic shrub, covered in fine grey hairs, stems rounded; blades ovate to diamond shaped, 5 — 15 long, acuminate with yellow dots below; flowers pale mauve or violet. It is a bush tea made for coughs and colds in Babados, and leaves are good healing wounds (Adegoke, 2009).Eupatorium odoratum is used as a traditional medicine in vietnam (Bharatiya, 2006) where its Vietnamese common name is “Cohoi”. While it has been widely considered as a weed by agriculturist (Glasby, 2004), the aqueous extracts and the decoction from the leaves of this plant have been used throughout Vietnam for the treatment of soft tissue wounds, burn wounds and skin infections.

The young leaves are crushed, and the resultant liquid can be used to treat skin infection and to stop cut wound from bleeding (Kowaro, 2006). In herbal medicine, leaf extracts with salt are used as a gargle for sore throats and colds. It is also used to scent aromatic baths (Oladunmoye 2007). Extracts of Christmas bush inhibit or kill Neisseria gonorrhoeae (the organism that cause gonorrhoea) in vitro (Okwu 2005) and to accelerate blood clotting (Edoga et al., 2003). Eupatorium odoratum adds copious amount of organic matter to the soil during fallows between cultivation and may reduce the population of nematodes (Ogunleye, 2003). It is also useful as a mulch for row crops (Sofowora, 2008). The Atlas of medicinal plants of middle America (Nuvinyi et al., 2004) reports that Eupatorium odoratumis used in Polynesia as a fish poison.

1.1 Statement of Problem

Eupatorium odoratum has acquired a reputation as a medicinal herb for a variety of ailments including malaria, fever, cough, cold and the aqueous leaf extract of the plant is used as an antiseptic for wound dressing. The decoction of the leaf was used as a cough remedy and the stem decoction can be used in pulmonary hemorrhage.


1.2 Objective of Study

  1. To isolate pseudomonas species from wound infection.
  2. To investigate the antimicrobial activity of Eupatorium odoratum against pseudomonas.

1.3 Significance of the Study

Eupatorium odoratum is a plant that has a variety of uses economically, medically and nutritionally, thus the reason for this study,. The work reveals that the Eupatorium odoratum are used orally against wounds and inflammation in traditional system.


1.4 Limitation of the Study

During this research work set back were encountered on the following areas;

Sourcing of information: Since health centers (Bishop Shanahan Nsukka) and institutes visited found it very hard to release already gathered.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …


How to Download the Complete PDF Material (Table of Contents, Abstract, Chapter 1-5, and References)


Above is a preview excerpt of the full study on “Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital”. The complete material, including all five chapters, is available for download upon request.

Defense Procedure for Science Laboratory Technology (SLT) Researchers


In preparation for defending a project or seminar on Studies on Antimicrobial Activities of Eupatorium Odoratum Extacts on Pseudomonas Specie Isolated From Wound Infection From Bishop Shanahan Hospital, it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


Conclude your defense by succinctly summarizing the study and offering insightful, evidence-based recommendations. A professional dress code, such as wearing a suit and tie, is vital to create a favorable impression and elevate your presentation.


During the question and answer segment, the External Examiner may pose questions pertaining to your research. If confronted with a challenging or irrelevant question, respond diplomatically with, “Sorry, Sir/Madam, the question asked is beyond the scope of my study.” Whenever possible, direct your answers back to your research findings to reinforce your expertise.


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