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Extraction and Analysis of Oil From Monodora Myristica (Ehuru Seed)

Extraction and Analysis of Oil From Monodora Myristica (Ehuru Seed)

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DEDICATION

This research material, titled “Extraction and Analysis of Oil From Monodora Myristica (Ehuru Seed)” is dedicated to God for His boundless grace and guidance. It is also a tribute to all computer enthusiasts whose contributions made my research journey smoother and enriched my documentation process, making the experience truly fulfilling.




ACKNOWLEDGEMENT

I am profoundly grateful to everyone who contributed to the successful completion of this project. I am especially grateful to my Supervisor (Name), the Head of Department (Name), and the Lecturers in the Department of Science Laboratory Technology (SLT) for their invaluable guidance and support. I also acknowledge the contributions of authors and scholars whose works on Extraction and Analysis of Oil From Monodora Myristica (Ehuru Seed) provided essential insights. Special thanks go to my study area (and any funding organizations, if applicable) for their financial assistance. I am equally thankful to stakeholders, including mentors, teachers, and colleagues, for their encouragement and support. Finally, I deeply appreciate my family and friends for their patience and unwavering support throughout this journey. Your contributions have been instrumental in making this research a reality.




ABSTRACT

Extraction and analysis of Monodora myristica (ehuru) oil was investigated, the result of the proximate analysis carried out on the seed reveals the presence of moisture, crude protein, crude fat, ash, crude fibre, and carbohydrate in the sample. The moisture, protein, fat, ash, crude fibre and carbohydrate content of Monodora myristica was found to be 4.0%, 11.9%, 11.3%, 4.6%, 8.7% and 42.5% respectively, fatty acid analysis of the oil revealed that Monodora myristica has the following fatty acid with the concentrations. Capric acid (0.36%), Palmitoleic acid (0.42%), Lauric acid (1.16%), myristic acid (0.63%) stearic acid (3.42%), Linolenic acid (39.81%), Linoleic acid (44.6%), capriclic acid (0.52%) and ?-linolenic acid (2.37%). The result inferred that if, consumed in sufficient amounts, the spices could contribute towards meeting human nutritional requirements for normal growth and adequate protection against disease arising from malnutrition.



Extraction and Analysis of Oil From Monodora Myristica (Ehuru Seed)


1.0 Introduction

Calabash nutmeg, Monodora myristica (Ehuru) is a tropical tree of the family Annonaceae or custard apple family of flowering plants. Other names of nutmeg include Jamaica nutmeg, African nutmeg, ehuru, ariwo, awerewa, ehiri, African orchid nutmeg. The calabash nutmeg tree grows naturally in evergreen forest from Liberia to Nigeria and Cameroon, Angola and also Uganda and West Kenya. Due to the slave trade in the 18th century, the tree was introduced to the Caribbean Island where it was established and become known as Jamaican nutmeg.

In 1897, the Monodora myristica was introduced to Bogor Botanical Gardebn, indonesia, where the trees flower on a regular basis but no fruit could yet be collected. Due to its large and orchid-like flowers, the tree is also grown as an ornamental and other food materials (Fournier et al, 1999).

The Monodora myristica tree can reach a height of 35m and 2m in diameter at breast height. It has a clear trunk and branches horizontally. The leaves are alternately arranged and drooping with the leaf blade being elliptical, oblory or broadcast towards the apex and tapering to the stalk. They are petiolate and can reach a size of up to 45 x 20cm (Burkill, 1997).

The flower appears at the base of new shoots and is singular, pendant, large and fragrant. The pedicel bears a leaf-like bract and can reach 20cm in length. The flower sepals are red-spotted, crisped and 2.5cm long. The corolla is formed of six petal of while the three outer reach a length of 10cm and show curled and red, green and yellow spots. The three inner petals are almost triangular and form a white yellowish cone which on the outer-side is red-spotted and green on the inside. The flower's stigmas become receptive before it's stemens mature and shed their pollen (protogymous). The flower is pollinated by insects (Fournier et al, 1999).

The fruit is a berry of 20cm diameter and 18 smooth, green and speherical and becomes woody. It is attached to a long stalk which is made up to 60cm long. Inside the fruit the numerous oblongoid, pale brown, 1.5cm long seeds are surrounded by a whitish fragrant pulp. The seed contains 5 — 9% of a colourless essential oil (Joseph, 2007).

The odour and taste of the Monodora myristica seed is similar to nutmeg and it is used as a popular spice in the West African cuisine. The fruits are collected from wild trees and the seeds are dried and sold whole or ground to be used in stews, soups, cakes and deserts. For medicinal purposes they are used as stimulants, stomachic, for headaches, sores and also as insect repellant. The seeds are also made into necklaces (Weiss, 2002).

The timbers obtained form Monodora myristica is hard but easy to work with and is used for carpentry, house fittings and joinery. In medicine, the bark is used in treatments of stomach aches. Febrile pains , eye disease and haemorrhoids (Weiss, 2002).

The essential oil that can be obtained from the leaves contains ß-caryophyllenc, ?-humulene and ?-pinene. The major compound found in the essential oil form the seed are ?-phellandrene, ?-pinene, myrcene, limonene and pinene (Burkil, 1997).

1.1 Objective of the Study

The objective of this research work is to extract and analyze the oil form the seed of Monodora myristica (ehuru seed).


1.2 Statement of Problem

Monodora myristica contains substantial ammonia of non-dryly oil and that has necessitated the need for a project work to be carried out on the extraction and analysis of oil form the seed of Monodora myristica.


1.3 Significance of the Study

The result of this research work will out line the procedure for extraction and analysis of the oil from the seed of Monodora myristica.


1.4 Limitation of the Study

This research work is limited to the extraction of oil form Monodora myristica seed and the analysis of the oil. The major problem encountered in the project work is lack of finance and as a group it is not easy to come together when required.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the conceputal review, theoretical framework, the review of related literature …

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Defense Procedure for Science Laboratory Technology Researchers


In preparation for defending a project or seminar on Extraction and Analysis of Oil From Monodora Myristica (Ehuru Seed), it is imperative that as a nursing student, you demonstrate comprehensive knowledge of your research. The defense process is structured to include presenting your work, answering questions, and illustrating its pertinence. Initially, provide a succinct yet thorough introduction to your research topic, emphasizing its importance and the objectives, ensuring that both the audience and the External Examiner can understand the scope of your study.


Prior to your defense, be thoroughly acquainted with your research abstract and the critical elements of Chapter One, including motivation for embarking on this research, problem statement, objectives, and significance. In Chapter Two, be ready to cite at least two references from the literature review. For Chapter Three, you should be equipped to discuss the methodologies, tools, and techniques utilized. In Chapter Four, defend your research by justifying the findings and linking them to your research objectives.


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