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Microbial Evaluation in the Unit Operation of the Processing of Cane Rat

Microbial Evaluation in the Unit Operation of the Processing of Cane Rat

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Reference ID: PS-85-TM

DEDICATION

This research material titled “Microbial Evaluation in the Unit Operation of the Processing of Cane Rat” is dedicated to God for his enabling grace, and to all computer enthusiasts who contributed to make life a pleasant experience during my research documentation.

ACKNOWLEDGEMENT

I extend my sincere gratitude to all those who contributed to the completion of this project. Special thanks to my Supervisor (Name of your Supervisor), the Head of Department (Name of your HOD), the Lecturers in the department of Animal Science, Book Authors and Profound Scholars of existing or related project material on “Microbial Evaluation in the Unit Operation of the Processing of Cane Rat” for their invaluable guidance, support, and expertise throughout the journey.

I am also grateful to your study area (mention any funding organizations, if applicable) for their financial assistance. This research would not have been possible without the encouragement and assistance of some stakeholders (mention any mentors, teachers, or colleagues). Additionally, I would like to acknowledge the understanding and patience of my family and friends during this endeavor. Your unwavering support has been a constant source of motivation. Thank you all for being part of this meaningful endeavor.


Microbial Evaluation in the Unit Operation of the Processing of Cane Rat

TABLE OF CONTENTS

PRELIMINARY PAGES


CHAPTER ONE

  • 1.0 Introduction
  • 1.1 Objectives of the Research

CHAPTER TWO

  • Literature Review
  • 2.1 Characteristics of the Cane Rat
  • 2.2 Distribution and Habitat of the Cane rat
  • 2.3 Physical Description
  • 2.4 Haematological and Biochemical Values in the Cane Rats
  • 2.5 Reasons for Losses in Captive Cane rats
  • 2.6 Gastrointestinal Helminthes
  • 2.7 Blood Protozoan Parasites
  • 2.8 Economic Potentials of the Cane rats
  • 2.9 Nutrients Derived from Cane rat Meat
  • 2.10 Processing of Cane Rat Meat
  • 2.11 Microbiology of the Cane rat Meat
  • 2.12 Factors that affect the Growth of Microorganisms in Cane Rat Meat
  • 2.12.1 Temperature
  • 2.12.2 pH
  • 2.12.3 Nutrients
  • 2.12.4 Water Availability
  • 2.12.5 Spoilage of Meat

CHAPTER THREE

  • Materials and Methods
  • 3.1 Sample Collection
  • 3.2 Preparation of Agar
  • 3.2.1 Potato Dextrose Agar (PDA) for mould evaluation
  • 3.2.2 Nutrient Agar (NA) for bacterial evaluation
  • 3.3 Microbiological analysis
  • 3.4 Characteristics of the bacterial isolates
  • 3.5 Identification of Bacterial and fungal Isolates
  • 3.6 Statistical analysis

CHAPTER FOUR

  • Results

CHAPTER FIVE

  • Discussion

CHAPTER SIX

  • Conclusion and Recommendation
  • 6.1 Conclusion
  • 6.2 Recommendation

REFERENCES

APPENDIX

ABSTRACT

This study was conducted to evaluate the microbial status of fresh and processed cane rat along the unit operation in some selected markets in Benin City, Nigeria. Fresh cane rats were obtained from the different markets which are from the three local government areas that is, Oredo, Egor and Ikpoba-Okha which make up Benin City, while the Control cane rat was processed in the Faculty of Agriculture laboratory. Direct plate method was done by taking swabs with a sterile swab stick at different points during the processing line was used for the microbial evaluation to determine the total bacteria and fungi (mould) count of the various cane rat samples. The result shows the total bacteria population from the various markets as follows: Control (191cfu/g), New Benin (199cfu/g), Uwa (192cfu/g) and Abico (220cfu/g). While the total fungal count, was in New Benin (41cfu/g). There was however, no fungal recorded for samples from other markets and the Control.

The mean microbial population along the processing line shows that the whole unprocessed cane rat has the highest microbial count of 60.75cfu/g, followed by after washing 59.50cfu/g, and after evisceration 47.00cfu/g and after scalding 33.25cfu/g. The analysis of variance showed that there was no significant difference (P


Microbial Evaluation in the Unit Operation of the Processing of Cane Rat

CHAPTER ONE

1.0 Introduction

Globally, wildlife has great potentials for meat production and serves as an important source of the highly desired animal protein to the people of Africa, both in urban areas and rural communities (Fonweban and Njiwe, 1990). The preference for such merit or the meat of commercially available game animals is widely accepted (Baptist and Mengah, 1986). However, with ever increasing human population and obvious protein shortage in Africa, there is the need for an exploration of other means to provide readily accepted meat or short terms basis.

Among the wild rodents, the cane rat, or cane rat or cane cutter is the most preferred (Goffey, 1981). Cane rat (Thryonomys swinderianus) is a wild hystricomophic rodent widely distributed in the African sub-region and exploited in most areas as a source of animal protein (NRC, 1991). Being the most preferred and one of the most expensive meats in West Africa including Nigeria, Togo, Benin, Ghana and Cote d' Voire (Baptist and Mensah, 1998; Asibey and Addo, 2000), it constitutes to both local and export earning of most West Africa countries (NRC, 1991; Yeboah and Adamu, 1995). To alleviate this problem, attempts are being made in the sub-regional to domesticate the grass cutter (NRC, 1991; Addo, 2002) and make it more readily available, gain economic benefit and also reduce the environmental destruction that accompanies its collection from the wild.

Efficiently producing grass cutters in captivity demands that adequate nutrition is provided to ensure high productivity. However, grass cutter especially in the dry season poses a major challenge to produce in Nigeria.

Despite the role cane rat rearing plays in the livelihood of most Nigerians and the contribution to the daily protein intake, if continued, cane rat meat cause a source of food-borne illnesses especially under the condition in which animals are handled, slaughtered, transported and sold in Nigerian markets. Prescott, et al., (2002) shown that food items especially meat, are not only of high nutritional value to those who consume them but often are ideal culture media for microbial growth, meat is one of the most perishable foods, indicates composition is ideal for growth of a wide range of spoilages bacteria (Mayr, et al., 2003).

Mukhopadhyay et al., (2009) also reported that fresh raw meat like cane rat meat has been implicated for a number of meat borne infections and intoxications in several countries. This is because both pathogenic and non-pathogenic organisms live in the gastro intestinal tract (GIT) of cane rat which can be transformed onto the meat under faulty and poor processing conditions.

The advocacy for domestication of cane rat becomes stronger as it accepts indoor housing with about 90% of animals acclimatizing to domestic housing within three months (Asibey and Addo, 2000), with the acceptability of the meat among Nigerians, regardless of their religious of faith and social status further underscores the importance of domesticating this animal.

Due to the insatiable demand for the cane rat meat, some people have resorted to use poisons as bait to catch wild cane rat and this poses a serious health threat.

The inadequate supply of animal protein in developing countries has been attributed to inadequate production and high cost of conventional sources of animals protein (poultry, goat, meat, beef, mutton and pork) and cane rat meat, hence, an average Nigerian consumes only about a quarter of his minimum daily protein requirement (Oke, et al., 2004). To this end there has been increase in the consumption of bush meat. This increase has bridged the supply and demand protein gap (Abulude, 2004).

1.1 Objectives of the Research

The objectives of this study are to:

  1. Determine the microbial status of fresh cane rat
  2. Determine the critical control points at which microbes are likely to contaminate the fresh cane rat along processing line.
  3. Isolate, identify and characterize the micro organisms present in the fresh cane rat during processing.

CHAPTER TWO

2.0 Literature Review

2.1 Introduction

This chapter focuses on the review of related literature. A literature review includes the current knowledge as well as theoretical and methodological contributions to a particular topic. It documents the state of the art with respect to the topic you are writing. It surveys the literature in the topic selected. In this research work the literature review includes the …

Summary Headlines for Microbial Evaluation in the Unit Operation of the Processing of Cane Rat



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